Gene description for Prdx2
Gene name peroxiredoxin 2
Gene symbol Prdx2
Other names/aliases AL022839
Band-8
NkefB
PRP
PrxII
TDX1
TPx
TPx-B
TR
TSA
Tdpx1
Torin
Species Mus musculus
 Database cross references - Prdx2
ExoCarta ExoCarta_21672
Vesiclepedia VP_21672
Entrez Gene 21672
UniProt Q61171  
 Prdx2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mov neuroglial cells 15210972    
Oligodendrocytes 21136642    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Prdx2
Molecular Function
    peroxidase activity GO:0004601 TAS
    protein binding GO:0005515 IPI
    thioredoxin peroxidase activity GO:0008379 IBA
    thioredoxin peroxidase activity GO:0008379 ISO
    thioredoxin peroxidase activity GO:0008379 ISS
    thioredoxin peroxidase activity GO:0008379 TAS
    selenium binding GO:0008430 TAS
    antioxidant activity GO:0016209 ISO
Biological Process
    defense response to tumor cell GO:0002357 IEA
    defense response to tumor cell GO:0002357 ISO
    respiratory burst involved in inflammatory response GO:0002536 IMP
    response to oxidative stress GO:0006979 IBA
    response to oxidative stress GO:0006979 ISO
    response to oxidative stress GO:0006979 TAS
    regulation of hydrogen peroxide metabolic process GO:0010310 IMP
    removal of superoxide radicals GO:0019430 IBA
    removal of superoxide radicals GO:0019430 IMP
    removal of superoxide radicals GO:0019430 ISO
    positive regulation of blood coagulation GO:0030194 IMP
    negative regulation of lipopolysaccharide-mediated signaling pathway GO:0031665 IMP
    negative regulation of NF-kappaB transcription factor activity GO:0032088 IMP
    response to lipopolysaccharide GO:0032496 IMP
    cellular response to oxidative stress GO:0034599 ISO
    T cell proliferation GO:0042098 IMP
    hydrogen peroxide metabolic process GO:0042743 IMP
    hydrogen peroxide catabolic process GO:0042744 IBA
    hydrogen peroxide catabolic process GO:0042744 IMP
    regulation of apoptotic process GO:0042981 ISO
    T cell homeostasis GO:0043029 IMP
    positive regulation of MAPK cascade GO:0043410 IMP
    negative regulation of neuron apoptotic process GO:0043524 ISO
    leukocyte activation GO:0045321 IBA
    cell redox homeostasis GO:0045454 IBA
    negative regulation of T cell differentiation GO:0045581 IMP
    thymus development GO:0048538 IMP
    extrinsic apoptotic signaling pathway GO:0097191 IMP
    negative regulation of extrinsic apoptotic signaling pathway GO:2001237 IMP
    negative regulation of extrinsic apoptotic signaling pathway in absence of ligand GO:2001240 IDA
Subcellular Localization
    mitochondrion GO:0005739 HDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
    myelin sheath GO:0043209 HDA
 Experiment description of studies that identified Prdx2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 39
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|HSC70|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
3
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
4
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Prdx2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ppif  
Co-fractionation Mus musculus
2 Kctd13  
Affinity Capture-MS Mus musculus
3 Rpl32 19951
Co-fractionation Mus musculus
4 Eed  
Affinity Capture-MS Mus musculus
5 Dlg4  
Affinity Capture-MS Mus musculus
6 Mapt  
Affinity Capture-MS Mus musculus
7 Atg16l1  
Affinity Capture-MS Mus musculus
8 Ran 19384
Co-fractionation Mus musculus
9 HSD17B10 3028
Affinity Capture-MS Homo sapiens
10 Fancd2  
Affinity Capture-MS Mus musculus
11 Kcnma1  
Affinity Capture-MS Mus musculus
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