Gene description for Tnc
Gene name tenascin C
Gene symbol Tnc
Other names/aliases AI528729
C130033P17Rik
Hxb
TN
TN-C
Ten
cytotactin
tenascin-C
Species Mus musculus
 Database cross references - Tnc
ExoCarta ExoCarta_21923
Vesiclepedia VP_21923
Entrez Gene 21923
UniProt Q80YX1  
 Tnc identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mov neuroglial cells 15210972    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Tnc
Molecular Function
    fibronectin binding GO:0001968 ISO
    integrin binding GO:0005178 ISO
    integrin binding GO:0005178 ISS
    extracellular matrix structural constituent GO:0005201 RCA
    syndecan binding GO:0045545 ISO
Biological Process
    regulation of cell growth GO:0001558 ISO
    morphogenesis of an epithelium GO:0002009 IMP
    cell adhesion GO:0007155 IEA
    negative regulation of cell adhesion GO:0007162 IEA
    negative regulation of cell adhesion GO:0007162 ISO
    neuromuscular junction development GO:0007528 IMP
    neuromuscular junction development GO:0007528 IMP
    positive regulation of cell population proliferation GO:0008284 IDA
    response to wounding GO:0009611 ISO
    response to mechanical stimulus GO:0009612 IEA
    positive regulation of gene expression GO:0010628 IDA
    positive regulation of gene expression GO:0010628 IMP
    peripheral nervous system axon regeneration GO:0014012 IMP
    peripheral nervous system axon regeneration GO:0014012 IMP
    regulation of cell adhesion GO:0030155 IBA
    regulation of cell adhesion GO:0030155 ISO
    regulation of cell migration GO:0030334 NAS
    neuron projection development GO:0031175 ISO
    odontogenesis of dentin-containing tooth GO:0042475 IEA
    response to ethanol GO:0045471 IEA
    regulation of inflammatory response GO:0050727 IMP
    bud outgrowth involved in lung branching GO:0060447 IEA
    bud outgrowth involved in lung branching GO:0060447 ISO
    mesenchymal-epithelial cell signaling involved in prostate gland development GO:0060739 IMP
    prostate gland epithelium morphogenesis GO:0060740 IMP
    cellular response to retinoic acid GO:0071300 IEA
    cellular response to vitamin D GO:0071305 IEA
    response to fibroblast growth factor GO:0071774 IEA
    cellular response to prostaglandin D stimulus GO:0071799 IEA
Subcellular Localization
    extracellular region GO:0005576 IDA
    extracellular region GO:0005576 TAS
    basement membrane GO:0005604 IDA
    interstitial matrix GO:0005614 IDA
    extracellular space GO:0005615 IBA
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 IDA
    collagen-containing extracellular matrix GO:0062023 ISO
    tenascin complex GO:0090733 IPI
    extracellular matrix of synaptic cleft GO:0098965 IEA
    perisynaptic extracellular matrix GO:0098966 EXP
    perisynaptic extracellular matrix GO:0098966 IBA
    perisynaptic extracellular matrix GO:0098966 IDA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified Tnc in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 39
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|HSC70|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Tnc
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tenm1  
Co-fractionation Mus musculus
2 Itga8  
Co-fractionation Mus musculus
3 Pgk1 18655
Co-fractionation Mus musculus
4 Kctd13  
Affinity Capture-MS Mus musculus
5 Ncan  
Co-fractionation Mus musculus
6 Clu  
Co-fractionation Mus musculus
7 Cilp  
Co-fractionation Mus musculus
8 Cntn1 12805
Co-fractionation Mus musculus
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