Gene description for Was
Gene name Wiskott-Aldrich syndrome homolog (human)
Gene symbol Was
Other names/aliases U42471
Wasp
Species Mus musculus
 Database cross references - Was
ExoCarta ExoCarta_22376
Vesiclepedia VP_22376
Entrez Gene 22376
UniProt P70315  
 Was identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Was
Molecular Function
    actin binding GO:0003779 IEA
    protein binding GO:0005515 IPI
    SH3 domain binding GO:0017124 IEA
    SH3 domain binding GO:0017124 ISO
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
    small GTPase binding GO:0031267 IEA
    small GTPase binding GO:0031267 ISO
    identical protein binding GO:0042802 IDA
    identical protein binding GO:0042802 ISO
    phospholipase binding GO:0043274 IEA
    phospholipase binding GO:0043274 ISO
Biological Process
    regulation of T cell antigen processing and presentation GO:0002625 IEA
    regulation of T cell antigen processing and presentation GO:0002625 ISO
    immune response GO:0006955 ISO
    regulation of actin polymerization or depolymerization GO:0008064 IEA
    regulation of actin polymerization or depolymerization GO:0008064 ISO
    actin polymerization or depolymerization GO:0008154 IDA
    regulation of lamellipodium assembly GO:0010591 IEA
    regulation of lamellipodium assembly GO:0010591 ISO
    endosomal transport GO:0016197 IDA
    actin filament polymerization GO:0030041 IDA
    actin filament polymerization GO:0030041 ISO
    actin filament-based movement GO:0030048 IDA
    Cdc42 protein signal transduction GO:0032488 IEA
    Cdc42 protein signal transduction GO:0032488 ISO
    T cell activation GO:0042110 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 ISS
    regulation of stress fiber assembly GO:0051492 ISO
    negative regulation of stress fiber assembly GO:0051497 IEA
    negative regulation of stress fiber assembly GO:0051497 ISO
    cellular response to type II interferon GO:0071346 IDA
    positive regulation of double-strand break repair via homologous recombination GO:1905168 ISO
    positive regulation of double-strand break repair via homologous recombination GO:1905168 ISS
    negative regulation of cell motility GO:2000146 IEA
    negative regulation of cell motility GO:2000146 ISO
    regulation of double-strand break repair via nonhomologous end joining GO:2001032 ISS
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    actin filament GO:0005884 IEA
    actin filament GO:0005884 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    cell-cell junction GO:0005911 IDA
    vesicle membrane GO:0012506 IDA
    site of double-strand break GO:0035861 ISO
    site of double-strand break GO:0035861 ISS
    phagocytic vesicle GO:0045335 IDA
 Experiment description of studies that identified Was in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Was
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Atg16l1  
Affinity Capture-MS Mus musculus
2 SH3KBP1 30011
Affinity Capture-Western Homo sapiens
3 Usp7  
Affinity Capture-MS Mus musculus
4 Zap70 22637
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
5 Dab1  
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
6 Dlg1 13383
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
7 Lck 16818
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
View the network image/svg+xml
 Pathways in which Was is involved
No pathways found





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