Gene description for Pi4ka
Gene name phosphatidylinositol 4-kinase, catalytic, alpha polypeptide
Gene symbol Pi4ka
Other names/aliases Pik4ca
Species Mus musculus
 Database cross references - Pi4ka
ExoCarta ExoCarta_224020
Vesiclepedia VP_224020
Entrez Gene 224020
 Pi4ka identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Pi4ka
Molecular Function
    1-phosphatidylinositol 4-kinase activity GO:0004430 IBA
    1-phosphatidylinositol 4-kinase activity GO:0004430 IMP
    1-phosphatidylinositol 4-kinase activity GO:0004430 ISO
    ATP binding GO:0005524 IEA
Biological Process
    myelination in peripheral nervous system GO:0022011 IMP
    actin cytoskeleton organization GO:0030036 IMP
    Schwann cell migration GO:0036135 IMP
    modulation by host of viral process GO:0044788 IEA
    modulation by host of viral process GO:0044788 ISO
    phosphatidylinositol phosphate biosynthetic process GO:0046854 IBA
    phosphatidylinositol phosphate biosynthetic process GO:0046854 IMP
    phosphatidylinositol-mediated signaling GO:0048015 IBA
    reorganization of cellular membranes to establish viral sites of replication GO:0140754 IEA
    reorganization of cellular membranes to establish viral sites of replication GO:0140754 ISO
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    Golgi-associated vesicle membrane GO:0030660 ISO
 Experiment description of studies that identified Pi4ka in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Pi4ka
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Syngap1  
Affinity Capture-MS Mus musculus
2 Nphp4  
Affinity Capture-MS Mus musculus
3 Sstr3  
Proximity Label-MS Mus musculus
4 Cyfip1 20430
Affinity Capture-MS Mus musculus
5 Kctd13  
Affinity Capture-MS Mus musculus
6 Atg5  
Affinity Capture-RNA Mus musculus
7 Ttc7b  
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
8 Efr3b  
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
View the network image/svg+xml



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