Gene description for A2m
Gene name alpha-2-macroglobulin
Gene symbol A2m
Other names/aliases A2mp
Species Mus musculus
 Database cross references - A2m
ExoCarta ExoCarta_232345
Vesiclepedia VP_232345
Entrez Gene 232345
UniProt Q6GQT1  
 A2m identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for A2m
Molecular Function
    protease binding GO:0002020 IBA
    protease binding GO:0002020 IEA
    protease binding GO:0002020 ISO
    endopeptidase inhibitor activity GO:0004866 IBA
    endopeptidase inhibitor activity GO:0004866 ISO
    serine-type endopeptidase inhibitor activity GO:0004867 IEA
    serine-type endopeptidase inhibitor activity GO:0004867 ISO
    signaling receptor binding GO:0005102 IEA
    signaling receptor binding GO:0005102 ISO
    protein binding GO:0005515 IPI
    growth factor binding GO:0019838 ISO
    enzyme binding GO:0019899 ISO
    interleukin-8 binding GO:0019959 IEA
    interleukin-8 binding GO:0019959 ISO
    interleukin-1 binding GO:0019966 IEA
    interleukin-1 binding GO:0019966 ISO
    peptidase inhibitor activity GO:0030414 TAS
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    tumor necrosis factor binding GO:0043120 IEA
    tumor necrosis factor binding GO:0043120 ISO
    calcium-dependent protein binding GO:0048306 IEA
    calcium-dependent protein binding GO:0048306 ISO
    brain-derived neurotrophic factor binding GO:0048403 IEA
    brain-derived neurotrophic factor binding GO:0048403 ISO
    nerve growth factor binding GO:0048406 IEA
    nerve growth factor binding GO:0048406 ISO
Biological Process
    luteinization GO:0001553 IEA
    negative regulation of complement activation, lectin pathway GO:0001869 IEA
    negative regulation of complement activation, lectin pathway GO:0001869 ISO
    acute inflammatory response to antigenic stimulus GO:0002438 IEA
    acute-phase response GO:0006953 IEA
    female pregnancy GO:0007565 IEA
    response to nutrient GO:0007584 IEA
    response to carbon dioxide GO:0010037 IEA
    response to prostaglandin E GO:0034695 IEA
    stem cell differentiation GO:0048863 IDA
    response to glucocorticoid GO:0051384 IEA
    embryonic liver development GO:1990402 IEA
Subcellular Localization
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IEA
    extracellular space GO:0005615 ISO
 Experiment description of studies that identified A2m in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for A2m
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Atp6v1a 11964
Co-fractionation Mus musculus
2 Fancd2  
Affinity Capture-MS Mus musculus
3 HSD17B10 3028
Affinity Capture-MS Homo sapiens
4 Glb1  
Co-fractionation Mus musculus
View the network image/svg+xml



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