Gene description for SASH1
Gene name SAM and SH3 domain containing 1
Gene symbol SASH1
Other names/aliases SH3D6A
dJ323M4
dJ323M4.1
Species Homo sapiens
 Database cross references - SASH1
ExoCarta ExoCarta_23328
Vesiclepedia VP_23328
Entrez Gene 23328
HGNC 19182
MIM 607955
UniProt O94885  
 SASH1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Endothelial cells 26027894    
 Gene ontology annotations for SASH1
Molecular Function
    G-protein alpha-subunit binding GO:0001965 IDA
    protein binding GO:0005515 IDA
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IDA
    mitogen-activated protein kinase kinase kinase binding GO:0031435 IBA
    mitogen-activated protein kinase kinase kinase binding GO:0031435 IDA
    molecular adaptor activity GO:0060090 IDA
Biological Process
    protein polyubiquitination GO:0000209 IDA
    positive regulation of endothelial cell migration GO:0010595 IDA
    regulation of epithelial cell migration GO:0010632 IMP
    positive regulation of lipopolysaccharide-mediated signaling pathway GO:0031666 IMP
    positive regulation of JUN kinase activity GO:0043507 IMP
    positive regulation of angiogenesis GO:0045766 IMP
    regulation of protein K63-linked ubiquitination GO:1900044 IDA
    positive regulation of p38MAPK cascade GO:1900745 IMP
    positive regulation of non-canonical NF-kappaB signal transduction GO:1901224 IBA
    positive regulation of non-canonical NF-kappaB signal transduction GO:1901224 IMP
    regulation of protein autoubiquitination GO:1902498 IDA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    protein-containing complex GO:0032991 IMP
 Experiment description of studies that identified SASH1 in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 226
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for SASH1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
2 EPHA2 1969
Proximity Label-MS Homo sapiens
3 SFN 2810
Affinity Capture-MS Homo sapiens
4 ESR1  
Affinity Capture-MS Homo sapiens
5 MCAM 4162
Proximity Label-MS Homo sapiens
6 IRAK4 51135
Affinity Capture-Western Homo sapiens
7 KHDRBS1 10657
Protein-peptide Homo sapiens
8 YWHAG 7532
Affinity Capture-MS Homo sapiens
9 IKBKB 3551
Affinity Capture-Western Homo sapiens
10 FOXP1 27086
Co-fractionation Homo sapiens
11 YWHAB 7529
Affinity Capture-MS Homo sapiens
12 YWHAZ 7534
Affinity Capture-MS Homo sapiens
13 IRAK1  
Affinity Capture-Western Homo sapiens
14 SKIL  
Two-hybrid Homo sapiens
15 CHUK 1147
Affinity Capture-Western Homo sapiens
16 YWHAQ 10971
Affinity Capture-MS Homo sapiens
17 SUV39H2 79723
Co-crystal Structure Homo sapiens
18 MYD88 4615
Affinity Capture-Western Homo sapiens
19 MAP3K7  
Affinity Capture-Western Homo sapiens
20 IKBKG 8517
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which SASH1 is involved
No pathways found





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