Gene description for PPWD1
Gene name peptidylprolyl isomerase domain and WD repeat containing 1
Gene symbol PPWD1
Other names/aliases -
Species Homo sapiens
 Database cross references - PPWD1
ExoCarta ExoCarta_23398
Vesiclepedia VP_23398
Entrez Gene 23398
HGNC 28954
UniProt Q96BP3  
 PPWD1 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for PPWD1
Molecular Function
    peptidyl-prolyl cis-trans isomerase activity GO:0003755 IBA
    peptidyl-prolyl cis-trans isomerase activity GO:0003755 IDA
    RNA polymerase II CTD heptapeptide repeat P3 isomerase activity GO:0140839 IEA
    RNA polymerase II CTD heptapeptide repeat P6 isomerase activity GO:0140840 IEA
Biological Process
    mRNA splicing, via spliceosome GO:0000398 IC
    regulation of transcription by RNA polymerase II GO:0006357 IEA
    protein folding GO:0006457 IBA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    nuclear body GO:0016604 IDA
    catalytic step 2 spliceosome GO:0071013 IDA
 Experiment description of studies that identified PPWD1 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PPWD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DDX6 1656
Co-fractionation Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 PTPRF 5792
Co-fractionation Homo sapiens
4 RBM39 9584
Affinity Capture-MS Homo sapiens
5 CPSF6 11052
Affinity Capture-MS Homo sapiens
6 TP53 7157
Affinity Capture-MS Homo sapiens
7 SEC13 6396
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 Bcas2  
Affinity Capture-MS Mus musculus
9 SNRPB 6628
Affinity Capture-MS Homo sapiens
10 ADRBK1 156
Co-fractionation Homo sapiens
11 SNRPA 6626
Affinity Capture-MS Homo sapiens
12 Ccdc12  
Affinity Capture-MS Mus musculus
13 TOP1 7150
Affinity Capture-MS Homo sapiens
14 CHRNA7  
Affinity Capture-MS Homo sapiens
15 PTBP3 9991
Co-fractionation Homo sapiens
16 PARK2  
Affinity Capture-MS Homo sapiens
17 FKBP5 2289
Affinity Capture-MS Homo sapiens
18 ZNF232  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 SF3A1 10291
Affinity Capture-MS Homo sapiens
20 CBX3 11335
Proximity Label-MS Homo sapiens
21 NUP50 10762
Proximity Label-MS Homo sapiens
22 ZMYM6  
Affinity Capture-MS Homo sapiens
23 ACOX1 51
Co-fractionation Homo sapiens
24 HSPA2 3306
Affinity Capture-MS Homo sapiens
25 NTRK1 4914
Affinity Capture-MS Homo sapiens
26 CIT 11113
Affinity Capture-MS Homo sapiens
27 HSPA1A 3303
Affinity Capture-MS Homo sapiens
28 KRAS 3845
Negative Genetic Homo sapiens
29 SNRPC 6631
Affinity Capture-MS Homo sapiens
30 AR 367
Proximity Label-MS Homo sapiens
31 SNRPF 6636
Affinity Capture-MS Homo sapiens
32 SSRP1 6749
Affinity Capture-MS Homo sapiens
33 RPA3 6119
Proximity Label-MS Homo sapiens
34 CEBPA  
Proximity Label-MS Homo sapiens
35 EED  
Affinity Capture-MS Homo sapiens
36 ME1 4199
Co-fractionation Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here