Gene description for FOLH1
Gene name folate hydrolase (prostate-specific membrane antigen) 1
Gene symbol FOLH1
Other names/aliases FGCP
FOLH
GCP2
GCPII
NAALAD1
NAALAdase
PSM
PSMA
mGCP
Species Homo sapiens
 Database cross references - FOLH1
ExoCarta ExoCarta_2346
Vesiclepedia VP_2346
Entrez Gene 2346
HGNC 3788
MIM 600934
UniProt Q04609  
 FOLH1 identified in sEVs derived from the following tissue/cell type
Prostate cancer cells 22723089    
Saliva 19199708    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for FOLH1
Molecular Function
    carboxypeptidase activity GO:0004180 IBA
    metallocarboxypeptidase activity GO:0004181 IDA
    protein binding GO:0005515 IPI
    peptidase activity GO:0008233 NAS
    dipeptidase activity GO:0016805 IEA
    metal ion binding GO:0046872 IEA
    Ac-Asp-Glu binding GO:1904492 IDA
    tetrahydrofolyl-poly(glutamate) polymer binding GO:1904493 IDA
Biological Process
    proteolysis GO:0006508 IDA
    proteolysis GO:0006508 NAS
    folic acid-containing compound metabolic process GO:0006760 IEA
    C-terminal protein deglutamylation GO:0035609 IDA
Subcellular Localization
    cytoplasm GO:0005737 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IDA
    membrane GO:0016020 NAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified FOLH1 in sEVs
1
Experiment ID 144
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name C4-2 - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
2
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
3
Experiment ID 196
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 197
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for FOLH1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NAALAD2 10003
Affinity Capture-MS Homo sapiens
2 MME 4311
Affinity Capture-MS Homo sapiens
3 AR 367
Affinity Capture-MS Homo sapiens
4 MTAP 4507
Two-hybrid Homo sapiens
5 CTH 1491
Co-fractionation Homo sapiens
6 RPS3 6188
Two-hybrid Homo sapiens
7 GLG1 2734
Affinity Capture-MS Homo sapiens
8 SLC2A8 29988
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which FOLH1 is involved
PathwayEvidenceSource
Aspartate and asparagine metabolism TAS Reactome
Metabolism TAS Reactome
Metabolism of amino acids and derivatives TAS Reactome





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