Gene description for ACAP2
Gene name ArfGAP with coiled-coil, ankyrin repeat and PH domains 2
Gene symbol ACAP2
Other names/aliases CENTB2
CNT-B2
Species Homo sapiens
 Database cross references - ACAP2
ExoCarta ExoCarta_23527
Vesiclepedia VP_23527
Entrez Gene 23527
HGNC 16469
MIM 607766
UniProt Q15057  
 ACAP2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for ACAP2
Molecular Function
    GTPase activator activity GO:0005096 IDA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    actin filament-based process GO:0030029 IDA
    endocytic recycling GO:0032456 ISS
    cellular response to nerve growth factor stimulus GO:1990090 ISS
Subcellular Localization
    ruffle GO:0001726 IDA
    endosome membrane GO:0010008 IBA
    endosome membrane GO:0010008 ISS
    membrane GO:0016020 HDA
 Experiment description of studies that identified ACAP2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ACAP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 INSIG1  
Affinity Capture-MS Homo sapiens
3 MBOAT7 79143
Affinity Capture-MS Homo sapiens
4 DENR 8562
Co-fractionation Homo sapiens
5 RAB35 11021
Proximity Label-MS Homo sapiens
6 GPAA1 8733
Affinity Capture-MS Homo sapiens
7 PDK3 5165
Affinity Capture-MS Homo sapiens
8 NDUFS1 4719
Affinity Capture-MS Homo sapiens
9 SMURF1 57154
Affinity Capture-MS Homo sapiens
10 NDUFA9 4704
Affinity Capture-MS Homo sapiens
11 YWHAE 7531
Affinity Capture-MS Homo sapiens
12 UFL1 23376
Affinity Capture-MS Homo sapiens
13 PTEN 5728
Affinity Capture-MS Homo sapiens
14 HSPB1 3315
Two-hybrid Homo sapiens
15 ARF6 382
Proximity Label-MS Homo sapiens
16 SNX27 81609
Affinity Capture-MS Homo sapiens
17 EHBP1 23301
Affinity Capture-MS Homo sapiens
18 APEX1 328
Affinity Capture-RNA Homo sapiens
19 CAPZB 832
Affinity Capture-MS Homo sapiens
20 ACAP3 116983
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 SOAT1 6646
Affinity Capture-MS Homo sapiens
22 Tgs1  
Affinity Capture-MS Mus musculus
23 RPA3 6119
Proximity Label-MS Homo sapiens
24 IFI16 3428
Affinity Capture-MS Homo sapiens
25 GDI1 2664
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
26 Naa10  
Affinity Capture-MS Mus musculus
27 PIGS 94005
Affinity Capture-MS Homo sapiens
28 RAB11FIP3  
Affinity Capture-Western Homo sapiens
29 PRMT1 3276
Affinity Capture-MS Homo sapiens
30 CYLD  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ACAP2 is involved
No pathways found





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