Gene description for RABGAP1
Gene name RAB GTPase activating protein 1
Gene symbol RABGAP1
Other names/aliases GAPCENA
TBC1D11
Species Homo sapiens
 Database cross references - RABGAP1
ExoCarta ExoCarta_23637
Vesiclepedia VP_23637
Entrez Gene 23637
HGNC 17155
MIM 615882
UniProt Q9Y3P9  
 RABGAP1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for RABGAP1
Molecular Function
    GTPase activator activity GO:0005096 IBA
    GTPase activator activity GO:0005096 IDA
    protein binding GO:0005515 IPI
    tubulin binding GO:0015631 TAS
    small GTPase binding GO:0031267 IBA
    small GTPase binding GO:0031267 IPI
Biological Process
    regulation of GTPase activity GO:0043087 IDA
    regulation of cilium assembly GO:1902017 IMP
Subcellular Localization
    centrosome GO:0005813 IEA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    microtubule associated complex GO:0005875 TAS
 Experiment description of studies that identified RABGAP1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RABGAP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RABGAP1L 9910
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
2 GABARAPL1 23710
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
3 GYPA  
Affinity Capture-MS Homo sapiens
4 ORC2  
Co-fractionation Homo sapiens
5 DISC1 27185
Two-hybrid Homo sapiens
6 KRT37 8688
Affinity Capture-MS Homo sapiens
7 KIF2A 3796
Co-fractionation Homo sapiens
8 RAB35 11021
Proximity Label-MS Homo sapiens
9 RAB6A 5870
Two-hybrid Homo sapiens
10 VIPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 HRASLS2  
Affinity Capture-MS Homo sapiens
12 CCDC68  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 BCAS2 10286
Affinity Capture-MS Homo sapiens
14 LAMTOR1 55004
Proximity Label-MS Homo sapiens
15 ECSCR  
Affinity Capture-MS Homo sapiens
16 RAB7A 7879
Proximity Label-MS Homo sapiens
17 WIPI2 26100
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 HMGB3 3149
Co-fractionation Homo sapiens
19 APEH 327
Co-fractionation Homo sapiens
20 CD79B 974
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 TEX35  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 RBMX 27316
Co-fractionation Homo sapiens
23 Carm1 59035
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
24 PFN1 5216
Proximity Label-MS Homo sapiens
25 DLK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 RAB9A 9367
Proximity Label-MS Homo sapiens
27 HSP90AA1 3320
Co-fractionation Homo sapiens
28 TSKS  
Affinity Capture-MS Homo sapiens
29 HOXC4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 LIMS1 3987
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 GJA1 2697
Proximity Label-MS Homo sapiens
32 LSM4 25804
Affinity Capture-MS Homo sapiens
33 C19orf38  
Affinity Capture-MS Homo sapiens
34 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
35 KXD1 79036
Affinity Capture-MS Homo sapiens
36 MANSC1 54682
Affinity Capture-MS Homo sapiens
37 COPE 11316
Co-fractionation Homo sapiens
38 TUFT1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
40 COPG1 22820
Co-fractionation Homo sapiens
41 PSMA1 5682
Co-fractionation Homo sapiens
42 Pxdc1  
Affinity Capture-MS Mus musculus
43 RAB4A 5867
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
44 POLR2C 5432
Proximity Label-MS Homo sapiens
45 KIR2DS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 HOXA4  
Affinity Capture-MS Homo sapiens
47 DDIT3 1649
Affinity Capture-MS Homo sapiens
48 MAP1LC3A 84557
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
49 FIS1 51024
Affinity Capture-MS Homo sapiens
50 PPP1R13B  
Affinity Capture-MS Homo sapiens
51 CARM1 10498
Phenotypic Suppression Homo sapiens
52 HSP90B1 7184
Co-fractionation Homo sapiens
53 PSMA5 5686
Co-fractionation Homo sapiens
54 TACSTD2 4070
Affinity Capture-MS Homo sapiens
55 VCP 7415
Affinity Capture-MS Homo sapiens
56 NTRK1 4914
Affinity Capture-MS Homo sapiens
57 NUF2  
Affinity Capture-MS Homo sapiens
58 RAB11A 8766
Proximity Label-MS Homo sapiens
59 SENP1  
Biochemical Activity Homo sapiens
60 KIF5B 3799
Co-fractionation Homo sapiens
61 OSBP 5007
Co-fractionation Homo sapiens
62 CORO1A 11151
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 TRIM11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 CCDC53 51019
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 SENP5  
Biochemical Activity Homo sapiens
66 HAX1  
Proximity Label-MS Homo sapiens
67 WASH3P  
Affinity Capture-MS Homo sapiens
68 AK1 203
Cross-Linking-MS (XL-MS) Homo sapiens
69 GGA1 26088
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 TMEM206 55248
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 SEC61B 10952
Affinity Capture-MS Homo sapiens
72 RPL17 6139
Cross-Linking-MS (XL-MS) Homo sapiens
73 STX6 10228
Proximity Label-MS Homo sapiens
74 TRIM16 10626
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which RABGAP1 is involved
PathwayEvidenceSource
Membrane Trafficking TAS Reactome
Rab regulation of trafficking TAS Reactome
TBC/RABGAPs TAS Reactome
Vesicle-mediated transport TAS Reactome





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