Gene description for PLA2G15
Gene name phospholipase A2, group XV
Gene symbol PLA2G15
Other names/aliases ACS
GXVPLA2
LLPL
LPLA2
LYPLA3
Species Homo sapiens
 Database cross references - PLA2G15
ExoCarta ExoCarta_23659
Vesiclepedia VP_23659
Entrez Gene 23659
HGNC 17163
MIM 609362
UniProt Q8NCC3  
 PLA2G15 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for PLA2G15
Molecular Function
    lysophospholipase activity GO:0004622 IEA
    protein binding GO:0005515 IPI
    phospholipid binding GO:0005543 TAS
    zinc ion binding GO:0008270 IDA
    O-acyltransferase activity GO:0008374 IBA
    O-acyltransferase activity GO:0008374 IDA
    O-acyltransferase activity GO:0008374 ISS
    phospholipase A1 activity GO:0008970 IDA
    phospholipase A1 activity GO:0008970 ISS
    acylglycerol O-acyltransferase activity GO:0016411 ISS
    calcium-dependent phospholipase A2 activity GO:0047498 IEA
    calcium-independent phospholipase A2 activity GO:0047499 IBA
    calcium-independent phospholipase A2 activity GO:0047499 IDA
    calcium-independent phospholipase A2 activity GO:0047499 ISS
    calcium-independent phospholipase A2 activity GO:0047499 TAS
    phosphatidylserine 1-acylhydrolase activity GO:0052739 IEA
Biological Process
    phospholipid metabolic process GO:0006644 TAS
    glycerophospholipid metabolic process GO:0006650 IDA
    diacylglycerol biosynthetic process GO:0006651 ISS
    phosphatidylserine metabolic process GO:0006658 IDA
    ceramide metabolic process GO:0006672 IBA
    ceramide metabolic process GO:0006672 IDA
    ceramide metabolic process GO:0006672 ISS
    fatty acid catabolic process GO:0009062 TAS
    phosphatidylcholine catabolic process GO:0034638 ISS
    phosphatidylethanolamine catabolic process GO:0046338 ISS
    phosphatidylcholine metabolic process GO:0046470 IBA
    phosphatidylcholine metabolic process GO:0046470 IDA
    phosphatidylglycerol metabolic process GO:0046471 IDA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    nucleoplasm GO:0005654 IDA
    lysosome GO:0005764 IBA
    lysosome GO:0005764 ISS
    lysosome GO:0005764 NAS
    membrane GO:0016020 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified PLA2G15 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PLA2G15
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IMMT 10989
Two-hybrid Homo sapiens
2 CCT6A 908
Affinity Capture-MS Homo sapiens
3 GGH 8836
Affinity Capture-MS Homo sapiens
4 CCT7 10574
Affinity Capture-MS Homo sapiens
5 TCP1 6950
Affinity Capture-MS Homo sapiens
6 SCGB2A1 4246
Affinity Capture-MS Homo sapiens
7 CCT2 10576
Affinity Capture-MS Homo sapiens
8 FBXO2 26232
Affinity Capture-MS Homo sapiens
9 FBXO6 26270
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 IGF2R 3482
Affinity Capture-MS Homo sapiens
11 CCT6B 10693
Affinity Capture-MS Homo sapiens
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