Gene description for RAB38
Gene name RAB38, member RAS oncogene family
Gene symbol RAB38
Other names/aliases NY-MEL-1
rrGTPbp
Species Homo sapiens
 Database cross references - RAB38
ExoCarta ExoCarta_23682
Vesiclepedia VP_23682
Entrez Gene 23682
HGNC 9776
MIM 606281
UniProt P57729  
 RAB38 identified in sEVs derived from the following tissue/cell type
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for RAB38
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 NAS
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 NAS
    GTP-dependent protein binding GO:0030742 IPI
    AP-2 adaptor complex binding GO:0035612 IDA
    AP-1 adaptor complex binding GO:0035650 IPI
    AP-3 adaptor complex binding GO:0035651 IPI
    BLOC-2 complex binding GO:0036461 IPI
Biological Process
    intracellular protein transport GO:0006886 IBA
    mitochondrion organization GO:0007005 IMP
    small GTPase-mediated signal transduction GO:0007264 NAS
    protein transport GO:0015031 NAS
    vesicle-mediated transport GO:0016192 IEA
    melanosome organization GO:0032438 IBA
    endosome to melanosome transport GO:0035646 IMP
    positive regulation of melanin biosynthetic process GO:0048023 IEA
    platelet dense granule organization GO:0060155 IEA
    protein localization to membrane GO:0072657 IMP
    phagosome acidification GO:0090383 IMP
    melanosome assembly GO:1903232 IDA
    melanosome assembly GO:1903232 IMP
    positive regulation of protein localization to cell periphery GO:1904377 IEA
    positive regulation of phosphatidylcholine biosynthetic process GO:2001247 IEA
Subcellular Localization
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    lysosome GO:0005764 IDA
    early endosome GO:0005769 IDA
    trans-Golgi network GO:0005802 IBA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IEA
    endomembrane system GO:0012505 IBA
    membrane GO:0016020 IDA
    phagocytic vesicle membrane GO:0030670 IEA
    early endosome lumen GO:0031905 IDA
    melanosome membrane GO:0033162 IDA
    melanosome membrane GO:0033162 TAS
    melanosome GO:0042470 IBA
    melanosome GO:0042470 IDA
    mitochondria-associated endoplasmic reticulum membrane contact site GO:0044233 IDA
    cell body GO:0044297 IEA
    phagocytic vesicle GO:0045335 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified RAB38 in sEVs
1
Experiment ID 254
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 255
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 256
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 257
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for RAB38
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APLNR  
Affinity Capture-MS Homo sapiens
2 SLC18A1  
Affinity Capture-MS Homo sapiens
3 ICAM2 3384
Affinity Capture-MS Homo sapiens
4 AGPAT1 10554
Affinity Capture-MS Homo sapiens
5 BTNL9  
Affinity Capture-MS Homo sapiens
6 LAMP3  
Affinity Capture-MS Homo sapiens
7 OPALIN  
Affinity Capture-MS Homo sapiens
8 CARKD 55739
Affinity Capture-MS Homo sapiens
9 MFSD4  
Affinity Capture-MS Homo sapiens
10 NPTN 27020
Affinity Capture-MS Homo sapiens
11 SLC25A22  
Affinity Capture-MS Homo sapiens
12 FPR1  
Affinity Capture-MS Homo sapiens
13 ST6GALNAC6 30815
Affinity Capture-MS Homo sapiens
14 AQP3  
Affinity Capture-MS Homo sapiens
15 DDX58 23586
Affinity Capture-RNA Homo sapiens
16 FFAR1  
Affinity Capture-MS Homo sapiens
17 RAB32 10981
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 APP 351
Reconstituted Complex Homo sapiens
19 VSIG4  
Affinity Capture-MS Homo sapiens
20 ALPI  
Affinity Capture-MS Homo sapiens
21 MBNL1 4154
Affinity Capture-MS Homo sapiens
22 GPR45  
Affinity Capture-MS Homo sapiens
23 PINK1  
Affinity Capture-MS Homo sapiens
24 PARP1 142
Proximity Label-MS Homo sapiens
25 PIGH  
Affinity Capture-MS Homo sapiens
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