Gene description for Cpne1
Gene name copine I
Gene symbol Cpne1
Other names/aliases 1810028N16Rik
mKIAA4108
Species Mus musculus
 Database cross references - Cpne1
ExoCarta ExoCarta_266692
Vesiclepedia VP_266692
Entrez Gene 266692
UniProt Q8C166  
 Cpne1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cpne1
Molecular Function
    phosphatidylserine binding GO:0001786 ISO
    endopeptidase activity GO:0004175 ISO
    calcium ion binding GO:0005509 ISO
    calcium ion binding GO:0005509 ISS
    protein binding GO:0005515 IPI
    calcium-dependent phospholipid binding GO:0005544 IBA
    calcium-dependent phospholipid binding GO:0005544 IEA
    identical protein binding GO:0042802 ISO
    NF-kappaB binding GO:0051059 ISO
Biological Process
    proteolysis GO:0006508 ISO
    negative regulation of gene expression GO:0010629 ISO
    regulation of canonical NF-kappaB signal transduction GO:0043122 IBA
    regulation of canonical NF-kappaB signal transduction GO:0043122 ISO
    negative regulation of DNA binding GO:0043392 ISO
    positive regulation of neuron differentiation GO:0045666 ISO
    positive regulation of neuron differentiation GO:0045666 ISS
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 ISO
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 ISS
    cellular response to calcium ion GO:0071277 IBA
    cellular response to calcium ion GO:0071277 ISO
    negative regulation of non-canonical NF-kappaB signal transduction GO:1901223 ISO
    positive regulation of tumor necrosis factor-mediated signaling pathway GO:1903265 IBA
    positive regulation of tumor necrosis factor-mediated signaling pathway GO:1903265 ISO
    neuron projection extension GO:1990138 ISO
    neuron projection extension GO:1990138 ISS
Subcellular Localization
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    membrane GO:0016020 ISO
    membrane GO:0016020 ISS
    nuclear membrane GO:0031965 ISO
 Experiment description of studies that identified Cpne1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cpne1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ube2o 217342
Co-fractionation Mus musculus
2 CPNE1 8904
Reconstituted Complex Homo sapiens
3 CPNE4 131034
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml



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