Gene description for GTF2F2
Gene name general transcription factor IIF, polypeptide 2, 30kDa
Gene symbol GTF2F2
Other names/aliases BTF4
RAP30
TF2F2
TFIIF
Species Homo sapiens
 Database cross references - GTF2F2
ExoCarta ExoCarta_2963
Vesiclepedia VP_2963
Entrez Gene 2963
HGNC 4653
MIM 189969
UniProt P13984  
 GTF2F2 identified in sEVs derived from the following tissue/cell type
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
 Gene ontology annotations for GTF2F2
Molecular Function
    DNA binding GO:0003677 IEA
    protein binding GO:0005515 IPI
    four-way junction helicase activity GO:0009378 IEA
    RNA polymerase II general transcription initiation factor activity GO:0016251 ISS
    double-stranded DNA helicase activity GO:0036121 IEA
    forked DNA-dependent helicase activity GO:0061749 IEA
    single-stranded 3'-5' DNA helicase activity GO:1990518 IEA
Biological Process
    transcription by RNA polymerase II GO:0006366 IMP
    transcription initiation at RNA polymerase II promoter GO:0006367 IBA
    transcription initiation at RNA polymerase II promoter GO:0006367 IDA
    transcription initiation at RNA polymerase II promoter GO:0006367 ISS
    transcription elongation by RNA polymerase II GO:0006368 IDA
    DNA duplex unwinding GO:0032508 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
Subcellular Localization
    nucleus GO:0005634 HDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    transcription factor TFIIF complex GO:0005674 IBA
    transcription factor TFIIF complex GO:0005674 IPI
    microtubule cytoskeleton GO:0015630 IDA
 Experiment description of studies that identified GTF2F2 in sEVs
1
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GTF2F2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GNL2 29889
Affinity Capture-MS Homo sapiens
2 CD3EAP  
Proximity Label-MS Homo sapiens
3 POLR2G 5436
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
4 GTF2F1 2962
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
5 PAEP  
Affinity Capture-MS Homo sapiens
6 SH3GL1 6455
Co-fractionation Homo sapiens
7 Gtf2b  
Affinity Capture-MS Mus musculus
8 MED29  
Affinity Capture-MS Homo sapiens
9 ANAPC15  
Affinity Capture-MS Homo sapiens
10 TMEM184A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 ZMYND19  
Two-hybrid Homo sapiens
12 BRD2  
Affinity Capture-MS Homo sapiens
13 TAF5  
Affinity Capture-Western Homo sapiens
14 CENPA  
Proximity Label-MS Homo sapiens
15 APEX1 328
Proximity Label-MS Homo sapiens
16 DDX23 9416
Proximity Label-MS Homo sapiens
17 TCTEX1D2  
Reconstituted Complex Homo sapiens
18 GTF2E2  
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
19 UBE2O 63893
Affinity Capture-MS Homo sapiens
20 FBXW7  
Affinity Capture-MS Homo sapiens
21 COL8A1 1295
Affinity Capture-MS Homo sapiens
22 GAGE2E 26749
Affinity Capture-MS Homo sapiens
23 ATG16L1 55054
Affinity Capture-MS Homo sapiens
24 POLR2C 5432
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
25 SERF2 10169
Affinity Capture-MS Homo sapiens
26 ASF1A 25842
Proximity Label-MS Homo sapiens
27 TNFRSF1B  
Affinity Capture-MS Homo sapiens
28 FAM177A1 283635
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 KIF21A 55605
Co-fractionation Homo sapiens
30 PRPS2 5634
Affinity Capture-MS Homo sapiens
31 TCEAL7  
Affinity Capture-MS Homo sapiens
32 POLR2K  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
33 AURKA 6790
Affinity Capture-MS Homo sapiens
34 SRRT 51593
Co-fractionation Homo sapiens
35 CTDP1 9150
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
36 TAF10 6881
Affinity Capture-Western Homo sapiens
37 WHSC1 7468
Proximity Label-MS Homo sapiens
38 SHMT1 6470
Co-fractionation Homo sapiens
39 SUPT5H 6829
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
40 MSX2  
Reconstituted Complex Homo sapiens
41 FHL2 2274
Two-hybrid Homo sapiens
42 BAG6 7917
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 ATF4  
Reconstituted Complex Homo sapiens
44 IFI27L1  
Affinity Capture-MS Homo sapiens
45 AKR7L 246181
Affinity Capture-MS Homo sapiens
46 UBR5 51366
Co-fractionation Homo sapiens
47 NTRK1 4914
Affinity Capture-MS Homo sapiens
48 RPL31 6160
Proximity Label-MS Homo sapiens
49 ARNT 405
Reconstituted Complex Homo sapiens
50 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
51 SUDS3  
Co-fractionation Homo sapiens
52 CEBPA  
Proximity Label-MS Homo sapiens
Protein-peptide Homo sapiens
53 GPRC5A 9052
Co-fractionation Homo sapiens
54 C1orf189  
Affinity Capture-MS Homo sapiens
55 POLR2B 5431
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
56 ANLN 54443
Affinity Capture-MS Homo sapiens
57 UBE2H 7328
Affinity Capture-MS Homo sapiens
58 POLR2H 5437
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
59 JPH3 57338
Affinity Capture-MS Homo sapiens
60 XPO1 7514
Affinity Capture-MS Homo sapiens
61 PSENEN  
Affinity Capture-MS Homo sapiens
62 HTATSF1 27336
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
63 POLR2E 5434
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
64 CBLN4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 POLR2A 5430
Co-purification Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
66 TBP  
Reconstituted Complex Homo sapiens
67 JUN 3725
Reconstituted Complex Homo sapiens
68 KCNE3  
Affinity Capture-MS Homo sapiens
69 LBH  
Affinity Capture-MS Homo sapiens
70 FOS 2353
Reconstituted Complex Homo sapiens
71 HECW2  
Affinity Capture-MS Homo sapiens
72 LTV1  
Co-fractionation Homo sapiens
73 SET 6418
Co-fractionation Homo sapiens
74 AHR 196
Reconstituted Complex Homo sapiens
75 RPL27A 6157
Reconstituted Complex Homo sapiens
76 Junb  
Affinity Capture-MS Mus musculus
77 MAD2L1 4085
Affinity Capture-MS Homo sapiens
78 BRD3 8019
Affinity Capture-MS Homo sapiens
79 TAF4 6874
Affinity Capture-Western Homo sapiens
80 SUMO1 7341
Affinity Capture-MS Homo sapiens
81 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
82 POLR2F  
Affinity Capture-MS Homo sapiens
83 POLR2J  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 TCEA1 6917
Reconstituted Complex Homo sapiens
85 MEN1 4221
Affinity Capture-MS Homo sapiens
86 EFTUD2 9343
Co-fractionation Homo sapiens
87 SUPT4H1  
Co-fractionation Homo sapiens
88 GTF2B 2959
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
89 C20orf197  
Affinity Capture-MS Homo sapiens
90 SPCS1 28972
Affinity Capture-MS Homo sapiens
91 ATG4B 23192
Affinity Capture-MS Homo sapiens
92 GTF2E1 2960
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
93 SERBP1 26135
Affinity Capture-MS Homo sapiens
94 KRAS 3845
Negative Genetic Homo sapiens
95 AR 367
Reconstituted Complex Homo sapiens
96 RPA3 6119
Proximity Label-MS Homo sapiens
97 OTX2  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which GTF2F2 is involved
PathwayEvidenceSource
Abortive elongation of HIV-1 transcript in the absence of Tat IEA Reactome
Disease IEA Reactome
Disease TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers IEA Reactome
ESR-mediated signaling TAS Reactome
Estrogen-dependent gene expression TAS Reactome
FGFR2 alternative splicing TAS Reactome
FGFR2 mutant receptor activation IEA Reactome
Formation of HIV elongation complex in the absence of HIV Tat IEA Reactome
Formation of HIV-1 elongation complex containing HIV-1 Tat TAS Reactome
Formation of HIV-1 elongation complex containing HIV-1 Tat IEA Reactome
Formation of RNA Pol II elongation complex TAS Reactome
Formation of the Early Elongation Complex TAS Reactome
Formation of the HIV-1 Early Elongation Complex IEA Reactome
Gene expression (Transcription) TAS Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway TAS Reactome
HIV elongation arrest and recovery IEA Reactome
HIV Infection IEA Reactome
HIV Infection TAS Reactome
HIV Life Cycle IEA Reactome
HIV Life Cycle TAS Reactome
HIV Transcription Elongation TAS Reactome
HIV Transcription Elongation IEA Reactome
HIV Transcription Initiation IEA Reactome
Infectious disease IEA Reactome
Infectious disease TAS Reactome
Influenza Infection TAS Reactome
Influenza Viral RNA Transcription and Replication TAS Reactome
Late Phase of HIV Life Cycle IEA Reactome
Late Phase of HIV Life Cycle TAS Reactome
Metabolism of RNA TAS Reactome
mRNA Capping TAS Reactome
mRNA Splicing TAS Reactome
mRNA Splicing - Major Pathway TAS Reactome
mRNA Splicing - Minor Pathway TAS Reactome
Pausing and recovery of HIV elongation IEA Reactome
Pausing and recovery of Tat-mediated HIV elongation IEA Reactome
Processing of Capped Intron-Containing Pre-mRNA TAS Reactome
RNA Pol II CTD phosphorylation and interaction with CE TAS Reactome
RNA Pol II CTD phosphorylation and interaction with CE during HIV infection IEA Reactome
RNA Polymerase II HIV Promoter Escape IEA Reactome
RNA Polymerase II Pre-transcription Events TAS Reactome
RNA Polymerase II Promoter Escape TAS Reactome
RNA polymerase II transcribes snRNA genes TAS Reactome
RNA polymerase II transcribes snRNA genes IEA Reactome
RNA Polymerase II Transcription TAS Reactome
RNA Polymerase II Transcription IEA Reactome
RNA Polymerase II Transcription Elongation TAS Reactome
RNA Polymerase II Transcription Initiation TAS Reactome
RNA Polymerase II Transcription Initiation And Promoter Clearance TAS Reactome
RNA Polymerase II Transcription Pre-Initiation And Promoter Opening TAS Reactome
Signal Transduction TAS Reactome
Signaling by FGFR TAS Reactome
Signaling by FGFR in disease IEA Reactome
Signaling by FGFR2 TAS Reactome
Signaling by FGFR2 IIIa TM IEA Reactome
Signaling by FGFR2 in disease IEA Reactome
Signaling by Nuclear Receptors TAS Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Tat-mediated elongation of the HIV-1 transcript TAS Reactome
Tat-mediated elongation of the HIV-1 transcript IEA Reactome
Tat-mediated HIV elongation arrest and recovery IEA Reactome
TP53 Regulates Transcription of DNA Repair Genes TAS Reactome
Transcription of the HIV genome IEA Reactome
Transcription of the HIV genome TAS Reactome
Transcriptional Regulation by TP53 TAS Reactome
Viral Infection Pathways IEA Reactome
Viral Infection Pathways TAS Reactome
Viral Messenger RNA Synthesis TAS Reactome





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