Gene description for Myl9
Gene name myosin, light chain 9, regulatory
Gene symbol Myl9
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Myl9
ExoCarta ExoCarta_296313
Vesiclepedia VP_296313
Entrez Gene 296313
UniProt Q64122  
 Myl9 identified in sEVs derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Myl9
Molecular Function
    structural constituent of cytoskeleton GO:0005200 ISO
    calcium ion binding GO:0005509 IEA
    myosin heavy chain binding GO:0032036 IBA
    myosin heavy chain binding GO:0032036 IEA
    myosin heavy chain binding GO:0032036 ISO
Biological Process
    myofibril assembly GO:0030239 IBA
    stress fiber assembly GO:0043149 ISO
    platelet aggregation GO:0070527 ISO
Subcellular Localization
    stress fiber GO:0001725 IBA
    stress fiber GO:0001725 IEA
    stress fiber GO:0001725 ISO
    stress fiber GO:0001725 ISO
    cytoplasm GO:0005737 IBA
    cell cortex GO:0005938 IEA
    myosin II complex GO:0016460 IBA
    myosin II complex GO:0016460 IEA
    myosin II complex GO:0016460 ISO
    myofibril GO:0030016 IBA
    Z disc GO:0030018 IEA
    Z disc GO:0030018 ISO
 Experiment description of studies that identified Myl9 in sEVs
1
Experiment ID 92
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|GAPDH|CD9|CD151
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
2
Experiment ID 93
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|GAPDH|CD9|CD151
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Myl9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
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