Gene description for MYLPF
Gene name myosin light chain, phosphorylatable, fast skeletal muscle
Gene symbol MYLPF
Other names/aliases HUMMLC2B
MRLC2
MYL11
Species Homo sapiens
 Database cross references - MYLPF
ExoCarta ExoCarta_29895
Vesiclepedia VP_29895
Entrez Gene 29895
HGNC 29824
UniProt Q96A32  
 MYLPF identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for MYLPF
Molecular Function
    calcium ion binding GO:0005509 IBA
    protein binding GO:0005515 IPI
    structural constituent of muscle GO:0008307 ISS
Biological Process
    muscle contraction GO:0006936 IMP
    immune response GO:0006955 IEA
    skeletal muscle tissue development GO:0007519 IBA
    skeletal muscle tissue development GO:0007519 ISS
    actin-myosin filament sliding GO:0033275 ISS
    muscle tissue morphogenesis GO:0060415 ISS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    lysosomal membrane GO:0005765 HDA
    cytosol GO:0005829 TAS
    muscle myosin complex GO:0005859 TAS
 Experiment description of studies that identified MYLPF in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MYLPF
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LATS1  
Affinity Capture-MS Homo sapiens
2 BTF3 689
Affinity Capture-MS Homo sapiens
3 RSPH6A  
Affinity Capture-MS Homo sapiens
4 TLE3  
Affinity Capture-MS Homo sapiens
5 UBXN2A  
Affinity Capture-MS Homo sapiens
6 TNFSF14  
Affinity Capture-MS Homo sapiens
7 TSPAN33 340348
Affinity Capture-MS Homo sapiens
8 ABTB2 25841
Affinity Capture-MS Homo sapiens
9 PPP2R4 5524
Affinity Capture-MS Homo sapiens
10 MECOM 2122
Affinity Capture-MS Homo sapiens
11 EZH2  
Affinity Capture-MS Homo sapiens
12 PPP2CB 5516
Affinity Capture-MS Homo sapiens
13 TP63  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MYLPF is involved
PathwayEvidenceSource
Muscle contraction TAS Reactome
Smooth Muscle Contraction TAS Reactome





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