Gene description for HLA-DRB5
Gene name major histocompatibility complex, class II, DR beta 5
Gene symbol HLA-DRB5
Other names/aliases HLA-DRB
Species Homo sapiens
 Database cross references - HLA-DRB5
ExoCarta ExoCarta_3127
Vesiclepedia VP_3127
Entrez Gene 3127
HGNC 4953
MIM 604776
UniProt Q30154  
 HLA-DRB5 identified in sEVs derived from the following tissue/cell type
B cells 12519789    
B cells 9685355    
B cells 20458337    
B cells 20458337    
B cells 20458337    
Breast milk 21235781    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Plasma 21235781    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Saliva 21235781    
T cells 11907077    
Thymus 23844026    
 Gene ontology annotations for HLA-DRB5
Molecular Function
    protein binding GO:0005515 IPI
    MHC class II protein complex binding GO:0023026 IBA
    peptide antigen binding GO:0042605 IBA
    peptide antigen binding GO:0042605 ISS
Biological Process
    adaptive immune response GO:0002250 IEA
    peptide antigen assembly with MHC class II protein complex GO:0002503 IBA
    antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0019886 IBA
    positive regulation of immune response GO:0050778 IBA
    positive regulation of T cell activation GO:0050870 IBA
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    lysosomal membrane GO:0005765 IBA
    lysosomal membrane GO:0005765 IDA
    lysosomal membrane GO:0005765 TAS
    plasma membrane GO:0005886 TAS
    ER to Golgi transport vesicle membrane GO:0012507 TAS
    transport vesicle membrane GO:0030658 TAS
    endocytic vesicle membrane GO:0030666 TAS
    clathrin-coated endocytic vesicle membrane GO:0030669 TAS
    late endosome membrane GO:0031902 IBA
    late endosome membrane GO:0031902 IDA
    trans-Golgi network membrane GO:0032588 TAS
    MHC class II protein complex GO:0042613 IBA
    MHC class II protein complex GO:0042613 ISS
    extracellular exosome GO:0070062 HDA
    lumenal side of endoplasmic reticulum membrane GO:0098553 TAS
 Experiment description of studies that identified HLA-DRB5 in sEVs
1
Experiment ID 11
MISEV standards
✘ Biophysical techniques
✔
HSC70|HSP90|MHCII|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [MALDI TOF]
Western blotting
PubMed ID 12519789    
Organism Homo sapiens
Experiment description Proteomic and biochemical analyses of human B cell-derived exosomes. Potential implications for their function and multivesicular body formation.
Authors "Wubbolts R, Leckie RS, Veenhuizen PT, Schwarzmann G, Mobius W, Hoernschemeyer J, Slot JW, Geuze HJ, Stoorvogel W"
Journal name JBC
Publication year 2003
Sample B cells
Sample name RN (HLA-DR15+)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
Thin layer chromatography
HPLC
2
Experiment ID 16
MISEV standards
✔
EM
Biophysical techniques
✔
MHCII|CD86|LAMP1|LAMP2|CD63|CD81|CD82|ICAM1|TFRC
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
Immunoelectron Microscopy
PubMed ID 9685355    
Organism Homo sapiens
Experiment description Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes.
Authors "Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ"
Journal name JBC
Publication year 1998
Sample B cells
Sample name RN (HLA-DR15+)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.12-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Immunoelectron Microscopy
3
Experiment ID 79
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 80
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
5
Experiment ID 81
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
6
Experiment ID 124
MISEV standards
✔
IEM|EM
Biophysical techniques
✔
HSC70|CD63|CD9|CD81
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 21235781    
Organism Homo sapiens
Experiment description "Human saliva, plasma and breast milk exosomes contain RNA: uptake by macrophages."
Authors "Lasser C, Alikhani VS, Ekstrom K, Eldh M, Paredes PT, Bossios A, Sjostrand M, Gabrielsson S, Lotvall J, Valadi H."
Journal name JTM
Publication year 2011
Sample Breast milk
Sample name Breast milk - Normal
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
Immunoelectron Microscopy
Western blotting
7
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 123
MISEV standards
✔
IEM|EM
Biophysical techniques
✔
CD63|CD9|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 21235781    
Organism Homo sapiens
Experiment description "Human saliva, plasma and breast milk exosomes contain RNA: uptake by macrophages."
Authors "Lasser C, Alikhani VS, Ekstrom K, Eldh M, Paredes PT, Bossios A, Sjostrand M, Gabrielsson S, Lotvall J, Valadi H."
Journal name JTM
Publication year 2011
Sample Plasma
Sample name Plasma - Normal
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
Immunoelectron Microscopy
Western blotting
11
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
14
Experiment ID 122
MISEV standards
✔
IEM|EM
Biophysical techniques
✔
CD63|CD9|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 21235781    
Organism Homo sapiens
Experiment description "Human saliva, plasma and breast milk exosomes contain RNA: uptake by macrophages."
Authors "Lasser C, Alikhani VS, Ekstrom K, Eldh M, Paredes PT, Bossios A, Sjostrand M, Gabrielsson S, Lotvall J, Valadi H."
Journal name JTM
Publication year 2011
Sample Saliva
Sample name Saliva - Normal
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
Immunoelectron Microscopy
Western blotting
15
Experiment ID 18
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 11907077    
Organism Homo sapiens
Experiment description TCR activation of human T cells induces the production of exosomes bearing the TCR/CD3/zeta complex.
Authors "Blanchard N, Lankar D, Faure F, Regnault A, Dumont C, Raposo G, Hivroz C"
Journal name JIMMU
Publication year 2002
Sample T cells
Sample name Jurkat
T cell blasts
Isolation/purification methods Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
16
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for HLA-DRB5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HSPA5 3309
Affinity Capture-MS Homo sapiens
2 ATP1A3 478
Affinity Capture-MS Homo sapiens
3 HSPA8 3312
Affinity Capture-MS Homo sapiens
4 HLA-DRA 3122
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 YWHAE 7531
Affinity Capture-MS Homo sapiens
6 POM121C  
Affinity Capture-MS Homo sapiens
7 MS4A1 931
Affinity Capture-MS Homo sapiens
8 PKM 5315
Affinity Capture-MS Homo sapiens
9 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
10 C17orf75 64149
Affinity Capture-MS Homo sapiens
11 UBR3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 HSP90AA1 3320
Affinity Capture-MS Homo sapiens
13 ABHD12 26090
Affinity Capture-MS Homo sapiens
14 TUBA1A 7846
Affinity Capture-MS Homo sapiens
15 MMGT1 93380
Affinity Capture-MS Homo sapiens
16 DUSP13  
Affinity Capture-MS Homo sapiens
17 ANXA11 311
Affinity Capture-MS Homo sapiens
18 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
19 STAT1 6772
Affinity Capture-MS Homo sapiens
20 EMC2 9694
Affinity Capture-MS Homo sapiens
21 CHEK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 ATP1B1 481
Affinity Capture-MS Homo sapiens
23 HLA-DRB1 3123
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 DGCR2 9993
Affinity Capture-MS Homo sapiens
25 HLA-A 3105
Affinity Capture-MS Homo sapiens
26 EPS15L1 58513
Affinity Capture-MS Homo sapiens
27 STAG2 10735
Affinity Capture-MS Homo sapiens
28 HLA-C 3107
Affinity Capture-MS Homo sapiens
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