Gene description for SLC29A2
Gene name solute carrier family 29 (equilibrative nucleoside transporter), member 2
Gene symbol SLC29A2
Other names/aliases DER12
ENT2
HNP36
Species Homo sapiens
 Database cross references - SLC29A2
ExoCarta ExoCarta_3177
Vesiclepedia VP_3177
Entrez Gene 3177
HGNC 11004
MIM 602110
UniProt Q14542  
 SLC29A2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 34887515    
 Gene ontology annotations for SLC29A2
Molecular Function
    neurotransmitter transmembrane transporter activity GO:0005326 IDA
    neurotransmitter transmembrane transporter activity GO:0005326 ISS
    nucleoside transmembrane transporter activity GO:0005337 IBA
    nucleoside transmembrane transporter activity GO:0005337 IDA
    nucleoside transmembrane transporter activity GO:0005337 TAS
    nucleobase transmembrane transporter activity GO:0015205 IDA
    adenine transmembrane transporter activity GO:0015207 IDA
    guanine transmembrane transporter activity GO:0015208 IDA
    uracil transmembrane transporter activity GO:0015210 IDA
    purine nucleoside transmembrane transporter activity GO:0015211 IDA
    purine nucleoside transmembrane transporter activity GO:0015211 ISS
    cytidine transmembrane transporter activity GO:0015212 IDA
    uridine transmembrane transporter activity GO:0015213 IDA
Biological Process
    neurotransmitter uptake GO:0001504 ISS
    neurotransmitter transport GO:0006836 IDA
    xenobiotic transmembrane transport GO:0006855 TAS
    nucleobase transport GO:0015851 IDA
    adenine transport GO:0015853 IBA
    adenine transport GO:0015853 IDA
    guanine transport GO:0015854 IBA
    nucleoside transport GO:0015858 IDA
    purine nucleoside transmembrane transport GO:0015860 IDA
    purine nucleoside transmembrane transport GO:0015860 ISS
    cytidine transport GO:0015861 IDA
    uridine transmembrane transport GO:0015862 IBA
    uridine transmembrane transport GO:0015862 IDA
    adenosine transport GO:0032238 IDA
    adenosine transport GO:0032238 ISS
    inosine transport GO:0035340 IDA
    hypoxanthine transport GO:0035344 IBA
    hypoxanthine transport GO:0035344 IDA
    thymine transport GO:0035364 IBA
    thymine transport GO:0035364 IDA
    pyrimidine-containing compound transmembrane transport GO:0072531 IDA
    transport across blood-brain barrier GO:0150104 NAS
    nucleoside transmembrane transport GO:1901642 IDA
    guanine transmembrane transport GO:1903716 IDA
    uracil transmembrane transport GO:1903791 IDA
    purine nucleobase transmembrane transport GO:1904823 IDA
Subcellular Localization
    nucleolus GO:0005730 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 TAS
    basolateral plasma membrane GO:0016323 IBA
    basolateral plasma membrane GO:0016323 IDA
    apical plasma membrane GO:0016324 IDA
 Experiment description of studies that identified SLC29A2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
 Protein-protein interactions for SLC29A2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ALOX5AP  
Two-hybrid Homo sapiens
2 CERS3  
Two-hybrid Homo sapiens
3 CREB3  
Two-hybrid Homo sapiens
4 SERINC2 347735
Two-hybrid Homo sapiens
5 TNFRSF10C  
Affinity Capture-MS Homo sapiens
6 GPR152  
Two-hybrid Homo sapiens
7 TMEM86B  
Two-hybrid Homo sapiens
8 TMEM242  
Two-hybrid Homo sapiens
9 ERGIC3 51614
Two-hybrid Homo sapiens
10 LAPTM4B  
Two-hybrid Homo sapiens
11 TMEM237 65062
Two-hybrid Homo sapiens
12 SLC10A6  
Two-hybrid Homo sapiens
13 TMEM33 55161
Two-hybrid Homo sapiens
14 GJA4  
Two-hybrid Homo sapiens
15 UBQLN1 29979
Two-hybrid Homo sapiens
16 CANX 821
Affinity Capture-MS Homo sapiens
17 ARF6 382
Proximity Label-MS Homo sapiens
18 PPAPDC1B 84513
Two-hybrid Homo sapiens
19 SLC10A1  
Two-hybrid Homo sapiens
20 TMX2 51075
Two-hybrid Homo sapiens
21 UBQLN2 29978
Two-hybrid Homo sapiens
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