Experiment description of studies that identified IGH in sEVs

1 Experiment ID 21 MISEV standards ✔ EM|IEM Biophysical techniques ✔ Alix|TSG101|HSP70|CD63 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry    PubMed ID 19837982     Organism Homo sapiens Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215. Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson" Journal name MCP Publication year 2009 Sample Colorectal cancer cells Sample name LIM1215 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.10-1.12 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [Orbitrap] Western blotting

2 Experiment ID 235 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-8 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

3 Experiment ID 126 MISEV standards ✘ Biophysical techniques ✔ GAPDH Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [LTQ-FT Ultra] PubMed ID Unpublished / Not applicable Organism Homo sapiens Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim" Journal name Mesenchymal Stem Cell Therapy Publication year 2011 Sample Mesenchymal stem cells Sample name huES9.E1 Isolation/purification methods HPLC Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Antobody array Mass spectrometry

Protein-protein interactions for IGH

Protein Interactor ExoCarta ID Identification method PubMed Species 1 C15orf39   Affinity Capture-MS Homo sapiens 2 SNX1 6642 Affinity Capture-MS Homo sapiens 3 SNX2 6643 Affinity Capture-MS Homo sapiens 4 SNCA 6622 Affinity Capture-MS Homo sapiens 5 TUBA4A 7277 Affinity Capture-MS Homo sapiens 6 IGHA1 3493 Affinity Capture-MS Homo sapiens 7 LONP2 83752 Affinity Capture-MS Homo sapiens 8 HSPA5 3309 Affinity Capture-MS Homo sapiens 9 ERLEC1 27248 Affinity Capture-MS Homo sapiens 10 SNX6 58533 Affinity Capture-MS Homo sapiens 11 KIAA1244   Affinity Capture-MS Homo sapiens 12 FOXP1 27086 Affinity Capture-MS Homo sapiens 13 NR1I3   Two-hybrid Homo sapiens 14 TUBB8 347688 Affinity Capture-MS Homo sapiens 15 N4BP2L2 10443 Affinity Capture-MS Homo sapiens View the network

Pathways in which IGH is involved

No pathways found