Gene description for NMNAT3
Gene name nicotinamide nucleotide adenylyltransferase 3
Gene symbol NMNAT3
Other names/aliases FKSG76
PNAT-3
PNAT3
Species Homo sapiens
 Database cross references - NMNAT3
ExoCarta ExoCarta_349565
Vesiclepedia VP_349565
Entrez Gene 349565
HGNC 20989
MIM 608702
UniProt Q96T66  
 NMNAT3 identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for NMNAT3
Molecular Function
    nicotinamide-nucleotide adenylyltransferase activity GO:0000309 IBA
    nicotinate-nucleotide adenylyltransferase activity GO:0004515 IBA
    nicotinate-nucleotide adenylyltransferase activity GO:0004515 IDA
    ATP binding GO:0005524 IEA
Biological Process
    nucleotide biosynthetic process GO:0009165 IC
    NAD biosynthetic process GO:0009435 IBA
    NAD biosynthetic process GO:0009435 IEA
    response to wounding GO:0009611 IEA
    response to tumor necrosis factor GO:0034612 IEA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 TAS
    axon GO:0030424 IEA
    neuronal cell body GO:0043025 IEA
 Experiment description of studies that identified NMNAT3 in sEVs
1
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for NMNAT3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HSPA8 3312
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 RBPMS 11030
Affinity Capture-MS Homo sapiens
3 HS2ST1 9653
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 SPTB 6710
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 DCX  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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