Gene description for IRF2
Gene name interferon regulatory factor 2
Gene symbol IRF2
Other names/aliases IRF-2
Species Homo sapiens
 Database cross references - IRF2
ExoCarta ExoCarta_3660
Vesiclepedia VP_3660
Entrez Gene 3660
HGNC 6117
MIM 147576
UniProt P14316  
 IRF2 identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for IRF2
Molecular Function
    RNA polymerase II transcription regulatory region sequence-specific DNA binding GO:0000977 IMP
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IMP
    DNA binding GO:0003677 IDA
    DNA-binding transcription factor activity GO:0003700 IMP
    protein binding GO:0005515 IPI
    sequence-specific double-stranded DNA binding GO:1990837 IDA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 TAS
    immune system process GO:0002376 IBA
    regulation of DNA-templated transcription GO:0006355 IMP
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    cell population proliferation GO:0008283 TAS
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    defense response to virus GO:0051607 IDA
Subcellular Localization
    chromatin GO:0000785 ISA
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    focal adhesion GO:0005925 IDA
 Experiment description of studies that identified IRF2 in sEVs
1
Experiment ID 237
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for IRF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RELA 5970
Reconstituted Complex Homo sapiens
2 BRD7  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
3 PPP3CA 5530
Affinity Capture-MS Homo sapiens
4 FASN 2194
Positive Genetic Homo sapiens
5 KAT2A  
Reconstituted Complex Homo sapiens
6 FKBP7 51661
Two-hybrid Homo sapiens
7 KAT2B  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
8 AP5Z1 9907
Affinity Capture-MS Homo sapiens
9 HCFC1 3054
Affinity Capture-MS Homo sapiens
10 PRDM1  
Co-localization Homo sapiens
11 CSNK2A1 1457
Biochemical Activity Homo sapiens
12 TRIM55  
Two-hybrid Homo sapiens
13 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
14 IRF2BPL 64207
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 APP 351
Reconstituted Complex Homo sapiens
16 FOXK1 221937
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 PRKACA 5566
Biochemical Activity Homo sapiens
18 IRF2BP1 26145
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 NCL 4691
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
20 IRF8  
Affinity Capture-Western Homo sapiens
21 FOXK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 FAM208B 54906
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 CTSS 1520
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 PPP3CB  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
25 NFKB1 4790
Reconstituted Complex Homo sapiens
26 IRF2BP2  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 HCFC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 IRF1  
Reconstituted Complex Homo sapiens
29 MDM2  
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
30 TRIM63  
Two-hybrid Homo sapiens
31 EP300 2033
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
32 ATG7 10533
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
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