Gene description for KPNA5
Gene name karyopherin alpha 5 (importin alpha 6)
Gene symbol KPNA5
Other names/aliases IPOA6
SRP6
Species Homo sapiens
 Database cross references - KPNA5
ExoCarta ExoCarta_3841
Vesiclepedia VP_3841
Entrez Gene 3841
HGNC 6398
MIM 604545
UniProt O15131  
 KPNA5 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for KPNA5
Molecular Function
    protein binding GO:0005515 IPI
    nuclear localization sequence binding GO:0008139 IBA
    nuclear import signal receptor activity GO:0061608 IBA
Biological Process
    protein import into nucleus GO:0006606 ISS
    NLS-bearing protein import into nucleus GO:0006607 IBA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IBA
    nucleoplasm GO:0005654 NAS
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 IDA
    cytosol GO:0005829 NAS
    cytosol GO:0005829 TAS
    NLS-dependent protein nuclear import complex GO:0042564 ISS
 Experiment description of studies that identified KPNA5 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for KPNA5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TWIST1  
Reconstituted Complex Homo sapiens
2 FBL 2091
Co-fractionation Homo sapiens
3 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
4 MYCN  
Affinity Capture-MS Homo sapiens
5 GATAD2B 57459
Affinity Capture-MS Homo sapiens
6 SPOP  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
7 RBM45  
Affinity Capture-MS Homo sapiens
8 IPO5 3843
Co-fractionation Homo sapiens
9 ZNF326 284695
Two-hybrid Homo sapiens
10 ANP32A 8125
Two-hybrid Homo sapiens
11 ZRANB2 9406
Affinity Capture-MS Homo sapiens
12 KPNA1 3836
Affinity Capture-MS Homo sapiens
13 SPOPL  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
14 LMNB1 4001
Two-hybrid Homo sapiens
15 NUP155 9631
Proximity Label-MS Homo sapiens
16 C12orf45  
Affinity Capture-MS Homo sapiens
17 IL32  
Two-hybrid Homo sapiens
18 KPNB1 3837
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
19 NOP56 10528
Affinity Capture-MS Homo sapiens
20 NMNAT1  
Two-hybrid Homo sapiens
21 CUL3 8452
Affinity Capture-MS Homo sapiens
22 TSEN54  
Two-hybrid Homo sapiens
23 RNMT 8731
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 RNGTT 8732
Affinity Capture-MS Homo sapiens
25 ATM 472
Two-hybrid Homo sapiens
26 DCPS 28960
Two-hybrid Homo sapiens
27 PTPN2 5771
Affinity Capture-MS Homo sapiens
28 MEPCE 56257
Affinity Capture-MS Homo sapiens
29 ADNP 23394
Affinity Capture-MS Homo sapiens
30 BRMS1 25855
Two-hybrid Homo sapiens
31 NIPBL 25836
Affinity Capture-MS Homo sapiens
32 KLHL7  
Affinity Capture-MS Homo sapiens
33 ANP32E 81611
Affinity Capture-MS Homo sapiens
34 RBBP4 5928
Reconstituted Complex Homo sapiens
35 AIPL1  
Affinity Capture-MS Homo sapiens
36 NXF1 10482
Affinity Capture-RNA Homo sapiens
37 PHB2 11331
Affinity Capture-Western Homo sapiens
38 NUP50 10762
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
39 CHD4 1108
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 POU6F2  
Two-hybrid Homo sapiens
41 ZBTB2 57621
Affinity Capture-MS Homo sapiens
42 MOV10 4343
Affinity Capture-RNA Homo sapiens
43 MYC  
Affinity Capture-MS Homo sapiens
44 SREK1IP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 PARK2  
Affinity Capture-MS Homo sapiens
46 MCM2 4171
Affinity Capture-MS Homo sapiens
47 RAB7A 7879
Proximity Label-MS Homo sapiens
48 LYAR 55646
Affinity Capture-MS Homo sapiens
49 RPS8 6202
Co-fractionation Homo sapiens
50 KAT5  
Two-hybrid Homo sapiens
51 ANP32B 10541
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
52 MEAF6  
Affinity Capture-MS Homo sapiens
53 MTA3 57504
Affinity Capture-MS Homo sapiens
54 CHD8 57680
Affinity Capture-MS Homo sapiens
55 PARP1 142
Proximity Label-MS Homo sapiens
56 E2F4  
Affinity Capture-MS Homo sapiens
57 KBTBD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 NUP153 9972
Affinity Capture-MS Homo sapiens
59 POLR3C 10623
Two-hybrid Homo sapiens
60 FOXE1  
Affinity Capture-MS Homo sapiens
61 C9orf72  
Affinity Capture-MS Homo sapiens
62 NRF1 4899
Two-hybrid Homo sapiens
63 RAN 5901
Co-fractionation Homo sapiens
View the network image/svg+xml



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