ExoCarta: Gene summary

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Gene description for LFNG
Gene name	LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Gene symbol	LFNG
Other names/aliases	SCDO3
Species	Homo sapiens
Database cross references - LFNG
ExoCarta	ExoCarta_3955
Vesiclepedia	VP_3955
Entrez Gene	3955
HGNC	6560
MIM	602576
UniProt	Q8NES3
LFNG identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells	19837982
Colorectal cancer cells	34887515

Gene ontology annotations for LFNG

Molecular Function
	O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity	GO:0033829	IBA
	O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity	GO:0033829	IDA
	O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity	GO:0033829	ISS
	O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity	GO:0033829	TAS
	metal ion binding	GO:0046872	IEA
Biological Process
	ovarian follicle development	GO:0001541	IEA
	somitogenesis	GO:0001756	ISS
	marginal zone B cell differentiation	GO:0002315	ISS
	compartment pattern specification	GO:0007386	IEA
	regulation of Notch signaling pathway	GO:0008593	IBA
	regulation of Notch signaling pathway	GO:0008593	ISS
	animal organ morphogenesis	GO:0009887	NAS
	regulation of somitogenesis	GO:0014807	IMP
	T cell differentiation	GO:0030217	ISS
	positive regulation of Notch signaling pathway	GO:0045747	IEA
	positive regulation of meiotic cell cycle	GO:0051446	IEA
	negative regulation of Notch signaling pathway involved in somitogenesis	GO:1902367	ISS
Subcellular Localization
	Golgi membrane	GO:0000139	TAS
	extracellular region	GO:0005576	NAS
	extracellular vesicle	GO:1903561	HDA

Experiment description of studies that identified LFNG in sEVs

Experiment ID

MISEV standards
✔ EM\|IEM	Biophysical techniques
✔ Alix\|TSG101\|HSP70\|CD63	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

19837982

Organism

Homo sapiens

Experiment description

Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.

Authors

"Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"

Journal name

MCP

Publication year

2009

Sample

Colorectal cancer cells

Sample name

LIM1215

Isolation/purification methods

Filtration
Ultracentrifugation
Sucrose density gradient

Flotation density

1.10-1.12 g/mL

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry [Orbitrap]
Western blotting

Experiment ID

1203

MISEV standards
✔ EM	Biophysical techniques
✔ SDCBP\|FLOT1\|CD9\|CD81\|CD63\|EPCAM\|GAPDH\|LAMP1\|TFRC\|CD151\|CD82\|LAMP2\|RAB35\|TSG101\|FLOT2\|RAB5B\|ICAM1\|RAB5A	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

34887515

Organism

Homo sapiens

Experiment description

Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets

Authors

"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."

Journal name

Nat Cell Biol

Publication year

2021

Sample

Colorectal cancer cells

Sample name

DiFi

Isolation/purification methods

Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation

Flotation density

Molecules identified in the study

Protein
miRNA

Methods used in the study

Western blotting
Mass spectrometry
RNA sequencing

Protein-protein interactions for LFNG

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

APP

351

Reconstituted Complex

Homo sapiens

CCT7

10574

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

CCT6A

908

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

WRAP73

49856

Affinity Capture-MS

Homo sapiens

TCP1

6950

Affinity Capture-MS

Homo sapiens

CCT3

7203

Affinity Capture-MS

Homo sapiens

CCT4

10575

Affinity Capture-MS

Homo sapiens

CCT6B

10693

Affinity Capture-MS

Homo sapiens

HSPA8

3312

Affinity Capture-MS

Homo sapiens

CCT2

10576

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

View the network

Pathways in which LFNG is involved

Pathway	Evidence	Source
Defective LFNG causes SCDO3	TAS	Reactome
Developmental Biology	IEA	Reactome
Disease	TAS	Reactome
Diseases associated with O-glycosylation of proteins	TAS	Reactome
Diseases of glycosylation	TAS	Reactome
Diseases of metabolism	TAS	Reactome
Formation of paraxial mesoderm	IEA	Reactome
Gastrulation	IEA	Reactome
Kidney development	IEA	Reactome
Nephron development	IEA	Reactome
Pre-NOTCH Expression and Processing	IEA	Reactome
Pre-NOTCH Processing in Golgi	IEA	Reactome
Signal Transduction	IEA	Reactome
Signaling by NOTCH	IEA	Reactome
Somitogenesis	IEA	Reactome

Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here