Gene description for MGAT5
Gene name mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase
Gene symbol MGAT5
Other names/aliases GNT-V
GNT-VA
Species Homo sapiens
 Database cross references - MGAT5
ExoCarta ExoCarta_4249
Vesiclepedia VP_4249
Entrez Gene 4249
HGNC 7049
MIM 601774
UniProt Q09328  
 MGAT5 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Mesenchymal stem cells 36408942    
Prostate cancer cells 25844599    
 Gene ontology annotations for MGAT5
Molecular Function
    protein phosphatase inhibitor activity GO:0004864 IDA
    alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase activity GO:0030144 IBA
    alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase activity GO:0030144 IDA
    alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase activity GO:0030144 NAS
    alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase activity GO:0030144 TAS
    manganese ion binding GO:0030145 IDA
Biological Process
    protein N-linked glycosylation GO:0006487 IBA
    protein N-linked glycosylation GO:0006487 IDA
    protein N-linked glycosylation via asparagine GO:0018279 IDA
    viral protein processing GO:0019082 TAS
    positive regulation of cell migration GO:0030335 IDA
    positive regulation of receptor signaling pathway via STAT GO:1904894 IDA
Subcellular Localization
    Golgi membrane GO:0000139 ISS
    Golgi membrane GO:0000139 TAS
    Golgi apparatus GO:0005794 IBA
    membrane GO:0016020 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified MGAT5 in sEVs
1
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for MGAT5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LY86  
Affinity Capture-MS Homo sapiens
2 LYZL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 SLC39A12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 GPM6A 2823
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 HSPA5 3309
Affinity Capture-MS Homo sapiens
6 TMEM106A  
Affinity Capture-MS Homo sapiens
7 ART3  
Affinity Capture-MS Homo sapiens
8 CEACAM8 1088
Affinity Capture-MS Homo sapiens
9 CCL3  
Affinity Capture-MS Homo sapiens
10 SLC39A4 55630
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 NRSN1  
Affinity Capture-MS Homo sapiens
12 C7orf34  
Affinity Capture-MS Homo sapiens
13 TMEM25  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 CFC1  
Affinity Capture-MS Homo sapiens
15 PLAUR 5329
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 PDGFRA 5156
Affinity Capture-MS Homo sapiens
17 PRG2  
Affinity Capture-MS Homo sapiens
18 MFAP4 4239
Affinity Capture-MS Homo sapiens
19 NIPAL1  
Affinity Capture-MS Homo sapiens
20 HM13 81502
Affinity Capture-MS Homo sapiens
21 SLC7A14  
Affinity Capture-MS Homo sapiens
22 LYZL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 IL5RA  
Affinity Capture-MS Homo sapiens
24 SFTPC  
Affinity Capture-MS Homo sapiens
25 SCN4A  
Affinity Capture-MS Homo sapiens
26 GAA 2548
Affinity Capture-MS Homo sapiens
27 HPN  
Affinity Capture-MS Homo sapiens
28 C1QB 713
Affinity Capture-MS Homo sapiens
29 BRICD5  
Affinity Capture-MS Homo sapiens
30 SDF2L1 23753
Affinity Capture-MS Homo sapiens
31 FIBIN  
Affinity Capture-MS Homo sapiens
32 SCGB2A2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 C1orf54  
Affinity Capture-MS Homo sapiens
34 ALPP 250
Affinity Capture-MS Homo sapiens
35 SCGB2A1 4246
Affinity Capture-MS Homo sapiens
36 DNAJC3 5611
Affinity Capture-MS Homo sapiens
37 TRAC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 NXPH3  
Affinity Capture-MS Homo sapiens
39 ISLR 3671
Affinity Capture-MS Homo sapiens
40 HLA-E 3133
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 PTCH1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 TRAV20  
Affinity Capture-MS Homo sapiens
43 TMEM59 9528
Affinity Capture-MS Homo sapiens
44 LYPD4  
Affinity Capture-MS Homo sapiens
45 MFF 56947
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 SLC39A8 64116
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 C3orf52  
Affinity Capture-MS Homo sapiens
48 TMPRSS13  
Affinity Capture-MS Homo sapiens
49 SLC7A1 6541
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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