Gene description for MMP15
Gene name matrix metallopeptidase 15 (membrane-inserted)
Gene symbol MMP15
Other names/aliases MT2-MMP
MTMMP2
SMCP-2
Species Homo sapiens
 Database cross references - MMP15
ExoCarta ExoCarta_4324
Vesiclepedia VP_4324
Entrez Gene 4324
HGNC 7161
MIM 602261
UniProt P51511  
 MMP15 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for MMP15
Molecular Function
    metalloendopeptidase activity GO:0004222 IBA
    metalloendopeptidase activity GO:0004222 TAS
    protein binding GO:0005515 IPI
    enzyme activator activity GO:0008047 TAS
    zinc ion binding GO:0008270 IEA
    metalloaminopeptidase activity GO:0070006 TAS
Biological Process
    proteolysis GO:0006508 TAS
    extracellular matrix disassembly GO:0022617 TAS
    extracellular matrix organization GO:0030198 IBA
    collagen catabolic process GO:0030574 IBA
    collagen catabolic process GO:0030574 TAS
    response to estradiol GO:0032355 IEA
    endodermal cell differentiation GO:0035987 IEP
    protein modification process GO:0036211 TAS
Subcellular Localization
    extracellular space GO:0005615 IBA
    plasma membrane GO:0005886 TAS
    extracellular matrix GO:0031012 IEA
 Experiment description of studies that identified MMP15 in sEVs
1
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MMP15
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UPK2 7379
Affinity Capture-MS Homo sapiens
2 CCL3  
Affinity Capture-MS Homo sapiens
3 ISG15 9636
Affinity Capture-MS Homo sapiens
4 TMEM59 9528
Affinity Capture-MS Homo sapiens
5 RTN3 10313
Affinity Capture-MS Homo sapiens
6 PRG2  
Affinity Capture-MS Homo sapiens
7 MFAP4 4239
Affinity Capture-MS Homo sapiens
8 PI15  
Affinity Capture-MS Homo sapiens
9 CIT 11113
Affinity Capture-MS Homo sapiens
10 LYZL1  
Affinity Capture-MS Homo sapiens
11 AQP3  
Affinity Capture-MS Homo sapiens
12 MOG  
Affinity Capture-MS Homo sapiens
13 IL5RA  
Affinity Capture-MS Homo sapiens
14 SFTPC  
Affinity Capture-MS Homo sapiens
15 SIRPD  
Affinity Capture-MS Homo sapiens
16 GPR45  
Affinity Capture-MS Homo sapiens
17 GORASP2 26003
Affinity Capture-MS Homo sapiens
18 TRPV3  
Affinity Capture-MS Homo sapiens
19 TMED7 51014
Affinity Capture-MS Homo sapiens
20 NCR3  
Affinity Capture-MS Homo sapiens
21 PCDHB3  
Affinity Capture-MS Homo sapiens
22 DKKL1  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here