Gene description for MXI1
Gene name MAX interactor 1, dimerization protein
Gene symbol MXI1
Other names/aliases MAD2
MXD2
MXI
bHLHc11
Species Homo sapiens
 Database cross references - MXI1
ExoCarta ExoCarta_4601
Vesiclepedia VP_4601
Entrez Gene 4601
HGNC 7534
MIM 600020
UniProt P50539  
 MXI1 identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for MXI1
Molecular Function
    RNA polymerase II transcription regulatory region sequence-specific DNA binding GO:0000977 IDA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    DNA-binding transcription repressor activity, RNA polymerase II-specific GO:0001227 IDA
    protein binding GO:0005515 IPI
    protein dimerization activity GO:0046983 IEA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    regulation of transcription by RNA polymerase II GO:0006357 IBA
Subcellular Localization
    chromatin GO:0000785 ISA
    nucleus GO:0005634 TAS
    nucleoplasm GO:0005654 IDA
    nucleolus GO:0005730 IDA
    cytosol GO:0005829 IDA
    RNA polymerase II transcription regulator complex GO:0090575 IDA
 Experiment description of studies that identified MXI1 in sEVs
1
Experiment ID 234
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for MXI1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KRTAP10-7  
Two-hybrid Homo sapiens
2 NOTCH2NL 388677
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
3 SMC3 9126
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
4 BTRC 8945
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 FBXW11  
Affinity Capture-MS Homo sapiens
6 CDC20 991
Affinity Capture-Western Homo sapiens
7 UBE2O 63893
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
8 PEAR1 375033
Two-hybrid Homo sapiens
9 MAX  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Luminescence Homo sapiens
10 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
11 KRTAP10-8  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
12 NBPF19  
Two-hybrid Homo sapiens
13 MDFI  
Two-hybrid Homo sapiens
14 BUB1B  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
15 ENTPD5 957
Two-hybrid Homo sapiens
16 KRTAP1-1  
Two-hybrid Homo sapiens
17 KRTAP10-3  
Two-hybrid Homo sapiens
18 SIN3B  
Affinity Capture-Western Homo sapiens
19 APP 351
Reconstituted Complex Homo sapiens
20 KRTAP5-7  
Two-hybrid Homo sapiens
21 Max  
Affinity Capture-MS Mus musculus
22 PRMT5 10419
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
23 CYSRT1 375791
Two-hybrid Homo sapiens
24 MXD3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 RPL23AP32 56969
Two-hybrid Homo sapiens
26 ZNF655  
Two-hybrid Homo sapiens
27 KRT40  
Two-hybrid Homo sapiens
28 CALCOCO2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
29 KRTAP1-3  
Two-hybrid Homo sapiens
30 KRTAP10-5  
Two-hybrid Homo sapiens
31 EFEMP2 30008
Two-hybrid Homo sapiens
32 KRTAP10-9  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which MXI1 is involved
No pathways found





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