Gene description for MYL4
Gene name myosin, light chain 4, alkali; atrial, embryonic
Gene symbol MYL4
Other names/aliases ALC1
AMLC
GT1
PRO1957
Species Homo sapiens
 Database cross references - MYL4
ExoCarta ExoCarta_4635
Vesiclepedia VP_4635
Entrez Gene 4635
HGNC 7585
MIM 160770
UniProt P12829  
 MYL4 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for MYL4
Molecular Function
    actin monomer binding GO:0003785 IBA
    actin monomer binding GO:0003785 IDA
    calcium ion binding GO:0005509 IEA
    myosin II heavy chain binding GO:0032038 NAS
    actin filament binding GO:0051015 IMP
Biological Process
    regulation of the force of heart contraction GO:0002026 IMP
    positive regulation of ATP-dependent activity GO:0032781 IMP
    cardiac muscle contraction GO:0060048 IBA
    cardiac muscle contraction GO:0060048 IMP
Subcellular Localization
    cytosol GO:0005829 TAS
    myosin II complex GO:0016460 IBA
    A band GO:0031672 IBA
    A band GO:0031672 IMP
 Experiment description of studies that identified MYL4 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MYL4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VCAM1 7412
Affinity Capture-MS Homo sapiens
2 CDC42 998
Affinity Capture-MS Homo sapiens
3 OSBPL11 114885
Affinity Capture-MS Homo sapiens
4 MYL1 4632
Affinity Capture-MS Homo sapiens
5 FN1 2335
Affinity Capture-MS Homo sapiens
6 UBE3B 89910
Affinity Capture-MS Homo sapiens
7 MYO18A 399687
Affinity Capture-MS Homo sapiens
8 CUL5 8065
Affinity Capture-MS Homo sapiens
9 ALB 213
Affinity Capture-MS Homo sapiens
10 PYROXD2 84795
Two-hybrid Homo sapiens
11 MYL3 4634
Affinity Capture-MS Homo sapiens
12 CAMKK2 10645
Affinity Capture-MS Homo sapiens
13 IQGAP3 128239
Affinity Capture-MS Homo sapiens
14 OSBPL9 114883
Affinity Capture-MS Homo sapiens
15 FILIP1  
Affinity Capture-MS Homo sapiens
16 ITGA4 3676
Affinity Capture-MS Homo sapiens
17 IQGAP1 8826
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MYL4 is involved
PathwayEvidenceSource
Muscle contraction TAS Reactome
Striated Muscle Contraction TAS Reactome





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