Gene description for NAGLU
Gene name N-acetylglucosaminidase, alpha
Gene symbol NAGLU
Other names/aliases MPS-IIIB
MPS3B
NAG
UFHSD
Species Homo sapiens
 Database cross references - NAGLU
ExoCarta ExoCarta_4669
Vesiclepedia VP_4669
Entrez Gene 4669
HGNC 7632
MIM 609701
UniProt P54802  
 NAGLU identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
Urine 15326289    
Urine 19056867    
Urine 21082674    
 Gene ontology annotations for NAGLU
Molecular Function
    alpha-N-acetylglucosaminidase activity GO:0004561 TAS
Biological Process
    ganglioside metabolic process GO:0001573 IEA
    microglial cell activation GO:0001774 IEA
    liver development GO:0001889 IEA
    endothelium development GO:0003158 IEA
    mitral valve morphogenesis GO:0003183 IEA
    left ventricular cardiac muscle tissue morphogenesis GO:0003220 IEA
    superoxide metabolic process GO:0006801 IEA
    autophagy GO:0006914 IEA
    cytoplasm organization GO:0007028 IEA
    Golgi organization GO:0007030 IEA
    lysosome organization GO:0007040 IEA
    nervous system development GO:0007399 TAS
    determination of adult lifespan GO:0008340 IEA
    response to wounding GO:0009611 IEA
    microglia differentiation GO:0014004 IEA
    protein processing GO:0016485 IEA
    nerve development GO:0021675 IEA
    cerebellar Purkinje cell layer development GO:0021680 IEA
    heparan sulfate proteoglycan catabolic process GO:0030200 TAS
    heparan sulfate proteoglycan metabolic process GO:0030201 IEA
    heparin metabolic process GO:0030202 IEA
    adult behavior GO:0030534 IEA
    hair follicle morphogenesis GO:0031069 IEA
    response to lipopolysaccharide GO:0032496 IEA
    collagen metabolic process GO:0032963 IEA
    toll-like receptor 4 signaling pathway GO:0034142 IEA
    response to disaccharide GO:0034285 IEA
    cellular response to oxidative stress GO:0034599 IEA
    maintenance of blood-brain barrier GO:0035633 IEA
    exploration behavior GO:0035640 IEA
    aorta morphogenesis GO:0035909 IEA
    hormone metabolic process GO:0042445 IEA
    middle ear morphogenesis GO:0042474 IEA
    amyloid precursor protein metabolic process GO:0042982 IEA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IEA
    locomotor rhythm GO:0045475 IEA
    retinal rod cell development GO:0046548 IEA
    astrocyte activation GO:0048143 IEA
    cardiac muscle cell development GO:0055013 IEA
    inner ear receptor cell development GO:0060119 IEA
    limb development GO:0060173 IEA
    multicellular organismal-level iron ion homeostasis GO:0060586 IEA
    motor behavior GO:0061744 IEA
    cellular response to organic cyclic compound GO:0071407 IEA
    sequestering of iron ion GO:0097577 IEA
    cell surface receptor signaling pathway via STAT GO:0097696 IEA
    vesicle tethering GO:0099022 IEA
    rod bipolar cell differentiation GO:1904389 IEA
    cone retinal bipolar cell differentiation GO:1904390 IEA
Subcellular Localization
    lysosome GO:0005764 TAS
    membrane GO:0016020 IEA
    lysosomal lumen GO:0043202 TAS
    extracellular exosome GO:0070062 HDA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified NAGLU in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 13
MISEV standards
✔
IEM
Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors "Pisitkun T, Shen RF, Knepper MA"
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
9
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
10
Experiment ID 102
MISEV standards
✔
IEM
Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21082674    
Organism Homo sapiens
Experiment description "Comprehensive analysis of low-abundance proteins in human urinary exosomes using peptide ligand library technology, peptide OFFGEL fractionation and nanoHPLC-chip-MS/MS."
Authors "Zhang Y, Li Y, Qiu F, Qiu Z."
Journal name ELEC
Publication year 2010
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Immunoelectron Microscopy
 Protein-protein interactions for NAGLU
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LY86  
Affinity Capture-MS Homo sapiens
2 LYZL2  
Affinity Capture-MS Homo sapiens
3 TEPP  
Affinity Capture-MS Homo sapiens
4 TMED9 54732
Co-fractionation Homo sapiens
5 C7orf34  
Affinity Capture-MS Homo sapiens
6 GGH 8836
Affinity Capture-MS Homo sapiens
7 LGALS9 3965
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 TMED10 10972
Co-fractionation Homo sapiens
9 ART3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 KLK15  
Affinity Capture-MS Homo sapiens
11 SCGB2A1 4246
Affinity Capture-MS Homo sapiens
12 LGI1  
Affinity Capture-MS Homo sapiens
13 CLGN 1047
Affinity Capture-MS Homo sapiens
14 SIAE 54414
Affinity Capture-MS Homo sapiens
15 TMEM87A 25963
Affinity Capture-MS Homo sapiens
16 LIPH 200879
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 DEFB109P1B  
Affinity Capture-MS Homo sapiens
18 TAZ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 DNASE2B  
Affinity Capture-MS Homo sapiens
20 FSTL4  
Affinity Capture-MS Homo sapiens
21 BRICD5  
Affinity Capture-MS Homo sapiens
22 CFC1  
Affinity Capture-MS Homo sapiens
23 PLAUR 5329
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 TMED4 222068
Co-fractionation Homo sapiens
25 PRG2  
Affinity Capture-MS Homo sapiens
26 MFAP4 4239
Affinity Capture-MS Homo sapiens
27 PI15  
Affinity Capture-MS Homo sapiens
28 LGALS1 3956
Affinity Capture-MS Homo sapiens
29 RLN1  
Affinity Capture-MS Homo sapiens
30 IFNE  
Affinity Capture-MS Homo sapiens
31 DNAJB9 4189
Affinity Capture-MS Homo sapiens
32 LYZL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 FBXO6 26270
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 OSBPL8 114882
Affinity Capture-MS Homo sapiens
35 P3H1 64175
Affinity Capture-MS Homo sapiens
36 NCR3  
Affinity Capture-MS Homo sapiens
37 SLAMF1 6504
Affinity Capture-MS Homo sapiens
38 INSL5  
Affinity Capture-MS Homo sapiens
39 DUSP13  
Affinity Capture-MS Homo sapiens
40 CD79A  
Affinity Capture-MS Homo sapiens
41 ALPPL2 251
Affinity Capture-MS Homo sapiens
42 C1QB 713
Affinity Capture-MS Homo sapiens
43 SIRPD  
Affinity Capture-MS Homo sapiens
44 IDS  
Affinity Capture-MS Homo sapiens
45 SUSD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 AVP  
Affinity Capture-MS Homo sapiens
47 NAAA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 FBXO2 26232
Affinity Capture-MS Homo sapiens
49 CRISP2  
Affinity Capture-MS Homo sapiens
50 SCGB2A2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 ALPP 250
Affinity Capture-MS Homo sapiens
52 IGF2R 3482
Affinity Capture-MS Homo sapiens
53 OSBPL5 114879
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 CEACAM8 1088
Affinity Capture-MS Homo sapiens
55 CDHR4  
Affinity Capture-MS Homo sapiens
56 KLK3  
Affinity Capture-MS Homo sapiens
57 EDN3  
Affinity Capture-MS Homo sapiens
58 ENPP7 339221
Affinity Capture-MS Homo sapiens
59 CBLN4  
Affinity Capture-MS Homo sapiens
60 UGT1A10  
Affinity Capture-MS Homo sapiens
61 IL5RA  
Affinity Capture-MS Homo sapiens
62 TRGV3  
Affinity Capture-MS Homo sapiens
63 CANX 821
Affinity Capture-MS Homo sapiens
64 SDF2L1 23753
Affinity Capture-MS Homo sapiens
65 LYPD4  
Affinity Capture-MS Homo sapiens
66 SKP1 6500
Affinity Capture-MS Homo sapiens
67 PRG3  
Affinity Capture-MS Homo sapiens
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