Gene description for NFATC4
Gene name nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4
Gene symbol NFATC4
Other names/aliases NF-AT3
NF-ATC4
NFAT3
Species Homo sapiens
 Database cross references - NFATC4
ExoCarta ExoCarta_4776
Vesiclepedia VP_4776
Entrez Gene 4776
HGNC 7778
MIM 602699
UniProt Q14934  
 NFATC4 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for NFATC4
Molecular Function
    transcription cis-regulatory region binding GO:0000976 ISS
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    DNA-binding transcription repressor activity, RNA polymerase II-specific GO:0001227 IGI
    protein binding GO:0005515 IPI
    peroxisome proliferator activated receptor binding GO:0042975 IEA
    sequence-specific double-stranded DNA binding GO:1990837 IDA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    branching involved in blood vessel morphogenesis GO:0001569 IEA
    transcription by RNA polymerase II GO:0006366 IEA
    inflammatory response GO:0006954 TAS
    heart development GO:0007507 IEA
    long-term memory GO:0007616 ISS
    intrinsic apoptotic signaling pathway in response to DNA damage GO:0008630 IEA
    negative regulation of Wnt signaling pathway GO:0030178 IEA
    brain-derived neurotrophic factor receptor signaling pathway GO:0031547 ISS
    positive regulation of tumor necrosis factor production GO:0032760 IEA
    calcineurin-NFAT signaling cascade GO:0033173 IBA
    cellular response to UV GO:0034644 IEA
    negative regulation of neuron apoptotic process GO:0043524 ISS
    positive regulation of neuron apoptotic process GO:0043525 IEA
    cellular respiration GO:0045333 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 IBA
    positive regulation of transcription by RNA polymerase II GO:0045944 ISS
    dendrite morphogenesis GO:0048813 IEA
    negative regulation of dendrite morphogenesis GO:0050774 IEA
    neuron apoptotic process GO:0051402 IEA
    synapse maturation GO:0060074 IEA
    long-term synaptic potentiation GO:0060291 ISS
    cellular response to lithium ion GO:0071285 IEA
    vascular associated smooth muscle cell development GO:0097084 IEA
    negative regulation of miRNA transcription GO:1902894 IGI
    negative regulation of synapse maturation GO:2000297 IEA
    positive regulation of apoptotic signaling pathway GO:2001235 IEA
Subcellular Localization
    chromatin GO:0000785 ISA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    transcription regulator complex GO:0005667 IBA
    cytosol GO:0005829 IDA
    nuclear speck GO:0016607 IDA
 Experiment description of studies that identified NFATC4 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NFATC4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MRPS12  
Proximity Label-MS Homo sapiens
2 NOP16 51491
Proximity Label-MS Homo sapiens
3 PPP3CA 5530
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 POLDIP3 84271
Proximity Label-MS Homo sapiens
5 SERPINA12  
Affinity Capture-MS Homo sapiens
6 JUP 3728
Two-hybrid Homo sapiens
7 HSPE1 3336
Affinity Capture-MS Homo sapiens
8 PWP1 11137
Proximity Label-MS Homo sapiens
9 NLGN3  
Two-hybrid Homo sapiens
10 GFAP 2670
Affinity Capture-MS Homo sapiens
11 YWHAZ 7534
Reconstituted Complex Homo sapiens
12 GRPEL1 80273
Proximity Label-MS Homo sapiens
13 CREBBP  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
14 ANKRD28 23243
Proximity Label-MS Homo sapiens
15 LPIN1 23175
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
16 APEX1 328
Affinity Capture-RNA Homo sapiens
17 JUN 3725
Affinity Capture-MS Homo sapiens
18 NR1I2  
Two-hybrid Homo sapiens
19 GTPBP4 23560
Proximity Label-MS Homo sapiens
20 TFAM 7019
Proximity Label-MS Homo sapiens
21 MAPK9 5601
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
22 SEC16A 9919
Proximity Label-MS Homo sapiens
23 HNRNPA1L2 144983
Affinity Capture-MS Homo sapiens
24 CCNG1  
Two-hybrid Homo sapiens
25 MAPK14 1432
Co-localization Homo sapiens
26 DBN1 1627
Affinity Capture-MS Homo sapiens
27 MAPK8 5599
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
28 ZYG11B  
Affinity Capture-MS Homo sapiens
29 BCL7A  
Proximity Label-MS Homo sapiens
30 CEBPA  
Affinity Capture-Western Homo sapiens
31 GPR22  
Two-hybrid Homo sapiens
32 PPP3CC 5533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NFATC4 is involved
No pathways found





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