Gene description for SLC11A2
Gene name solute carrier family 11 (proton-coupled divalent metal ion transporter), member 2
Gene symbol SLC11A2
Other names/aliases DCT1
DMT1
NRAMP2
Species Homo sapiens
 Database cross references - SLC11A2
ExoCarta ExoCarta_4891
Vesiclepedia VP_4891
Entrez Gene 4891
HGNC 10908
MIM 600523
UniProt P49281  
 SLC11A2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for SLC11A2
Molecular Function
    copper ion transmembrane transporter activity GO:0005375 IDA
    iron ion transmembrane transporter activity GO:0005381 TAS
    manganese ion transmembrane transporter activity GO:0005384 IBA
    manganese ion transmembrane transporter activity GO:0005384 IDA
    zinc ion transmembrane transporter activity GO:0005385 IDA
    zinc ion transmembrane transporter activity GO:0005385 IDA
    protein binding GO:0005515 IPI
    calcium ion transmembrane transporter activity GO:0015085 IDA
    cadmium ion transmembrane transporter activity GO:0015086 IBA
    cadmium ion transmembrane transporter activity GO:0015086 IDA
    cobalt ion transmembrane transporter activity GO:0015087 IBA
    cobalt ion transmembrane transporter activity GO:0015087 IDA
    ferrous iron transmembrane transporter activity GO:0015093 IBA
    ferrous iron transmembrane transporter activity GO:0015093 IDA
    ferrous iron transmembrane transporter activity GO:0015093 IMP
    ferrous iron transmembrane transporter activity GO:0015093 NAS
    lead ion transmembrane transporter activity GO:0015094 IBA
    lead ion transmembrane transporter activity GO:0015094 IDA
    nickel cation transmembrane transporter activity GO:0015099 IBA
    nickel cation transmembrane transporter activity GO:0015099 IDA
    vanadium ion transmembrane transporter activity GO:0015100 IDA
    solute:proton symporter activity GO:0015295 IDA
    inorganic cation transmembrane transporter activity GO:0022890 IGI
    cadmium ion binding GO:0046870 IBA
    cadmium ion binding GO:0046870 IDA
    transition metal ion transmembrane transporter activity GO:0046915 IDA
    chromium ion transmembrane transporter activity GO:0070835 IDA
    retromer complex binding GO:1905394 IDA
Biological Process
    response to hypoxia GO:0001666 IEP
    detection of oxygen GO:0003032 IEP
    detection of oxygen GO:0003032 IEP
    heme biosynthetic process GO:0006783 IEA
    cobalt ion transport GO:0006824 IDA
    cobalt ion transport GO:0006824 IEA
    copper ion transport GO:0006825 IDA
    iron ion transport GO:0006826 IDA
    manganese ion transport GO:0006828 IBA
    manganese ion transport GO:0006828 IDA
    intracellular iron ion homeostasis GO:0006879 TAS
    learning or memory GO:0007611 IEA
    response to iron ion GO:0010039 IEP
    response to iron ion GO:0010039 IEP
    nickel cation transport GO:0015675 IDA
    vanadium ion transport GO:0015676 IDA
    vanadium ion transport GO:0015676 IEA
    lead ion transport GO:0015692 IDA
    iron import into cell GO:0033212 IDA
    iron import into cell GO:0033212 NAS
    cellular response to oxidative stress GO:0034599 IDA
    iron ion transmembrane transport GO:0034755 IBA
    iron ion transmembrane transport GO:0034755 IDA
    iron ion transmembrane transport GO:0034755 IEA
    copper ion transmembrane transport GO:0035434 IEA
    nickel cation transmembrane transport GO:0035444 IEA
    dendrite morphogenesis GO:0048813 IEA
    erythrocyte development GO:0048821 IEA
    multicellular organismal-level iron ion homeostasis GO:0060586 IMP
    cadmium ion transmembrane transport GO:0070574 IDA
    manganese ion transmembrane transport GO:0071421 IEA
    zinc ion transmembrane transport GO:0071577 IEA
    proton transmembrane transport GO:1902600 IEA
    positive regulation of cysteine-type endopeptidase activity GO:2001056 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial outer membrane GO:0005741 IEA
    lysosome GO:0005764 IDA
    lysosomal membrane GO:0005765 HDA
    lysosomal membrane GO:0005765 IBA
    lysosomal membrane GO:0005765 IDA
    early endosome GO:0005769 IDA
    late endosome GO:0005770 IDA
    vacuole GO:0005773 IMP
    Golgi apparatus GO:0005794 IEA
    trans-Golgi network GO:0005802 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IMP
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IDA
    endosome membrane GO:0010008 IBA
    membrane GO:0016020 HDA
    apical plasma membrane GO:0016324 IDA
    cytoplasmic vesicle GO:0031410 IDA
    brush border membrane GO:0031526 ISS
    early endosome membrane GO:0031901 IEA
    late endosome membrane GO:0031902 IDA
    apical part of cell GO:0045177 IDA
    basal part of cell GO:0045178 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    recycling endosome GO:0055037 IDA
    recycling endosome membrane GO:0055038 IEA
    paraferritin complex GO:0070826 IDA
    extracellular vesicle GO:1903561 IDA
 Experiment description of studies that identified SLC11A2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for SLC11A2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SPPL2B 56928
Affinity Capture-MS Homo sapiens
2 VPS35 55737
Reconstituted Complex Homo sapiens
3 PMPCB 9512
Affinity Capture-MS Homo sapiens
4 GPR89A  
Affinity Capture-MS Homo sapiens
5 NEDD4 4734
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
6 CANX 821
Affinity Capture-MS Homo sapiens
7 LAMP3  
Proximity Label-MS Homo sapiens
8 NDFIP2 54602
Affinity Capture-Western Homo sapiens
9 NDFIP1 80762
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
10 PXMP2  
Affinity Capture-MS Homo sapiens
11 TVP23C  
Affinity Capture-MS Homo sapiens
12 LGALS3 3958
Affinity Capture-MS Homo sapiens
13 LAMP2 3920
Proximity Label-MS Homo sapiens
14 PARK2  
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
15 CYP20A1 57404
Affinity Capture-MS Homo sapiens
16 HACD2 201562
Affinity Capture-MS Homo sapiens
17 ND1  
Affinity Capture-MS Homo sapiens
18 RAB9A 9367
Proximity Label-MS Homo sapiens
19 SLC25A17 10478
Affinity Capture-MS Homo sapiens
20 ATP2B4 493
Affinity Capture-MS Homo sapiens
21 RER1 11079
Affinity Capture-MS Homo sapiens
22 UQCRH 7388
Affinity Capture-MS Homo sapiens
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