Gene description for OAZ1
Gene name ornithine decarboxylase antizyme 1
Gene symbol OAZ1
Other names/aliases AZI
OAZ
Species Homo sapiens
 Database cross references - OAZ1
ExoCarta ExoCarta_4946
Vesiclepedia VP_4946
Entrez Gene 4946
HGNC 8095
MIM 601579
UniProt P54368  
 OAZ1 identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for OAZ1
Molecular Function
    protein binding GO:0005515 IPI
    ornithine decarboxylase inhibitor activity GO:0008073 IBA
    ornithine decarboxylase inhibitor activity GO:0008073 IDA
    ornithine decarboxylase inhibitor activity GO:0008073 ISS
Biological Process
    polyamine biosynthetic process GO:0006596 IEA
    positive regulation of protein catabolic process GO:0045732 IBA
    positive regulation of protein catabolic process GO:0045732 IDA
    positive regulation of protein catabolic process GO:0045732 ISS
    viral translational frameshifting GO:0075523 IEA
    positive regulation of intracellular protein transport GO:0090316 ISS
Subcellular Localization
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified OAZ1 in sEVs
1
Experiment ID 234
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
4
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for OAZ1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 ATXN1 6310
Two-hybrid Homo sapiens
3 FASN 2194
Negative Genetic Homo sapiens
4 UPF1 5976
Affinity Capture-MS Homo sapiens
5 LENG8  
Affinity Capture-MS Homo sapiens
6 ACTA2 59
Affinity Capture-MS Homo sapiens
7 NLGN3  
Two-hybrid Homo sapiens
8 TAF1B  
Affinity Capture-MS Homo sapiens
9 AZIN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 PSMB4 5692
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
11 ODC1 4953
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
12 EGFR 1956
PCA Homo sapiens
13 SRA1 10011
Affinity Capture-MS Homo sapiens
14 RNF20 56254
Affinity Capture-MS Homo sapiens
15 PIAS4  
Two-hybrid Homo sapiens
16 AZIN1 51582
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
17 DDX58 23586
Affinity Capture-RNA Homo sapiens
18 TP73  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
19 GPATCH4 54865
Affinity Capture-MS Homo sapiens
20 SMAD1 4086
Two-hybrid Homo sapiens
21 CAMSAP2  
Affinity Capture-MS Homo sapiens
22 RAB7A 7879
Affinity Capture-MS Homo sapiens
23 HTR2B  
Two-hybrid Homo sapiens
24 GBE1 2632
Affinity Capture-MS Homo sapiens
25 LDLR 3949
Negative Genetic Homo sapiens
26 LCN2 3934
Affinity Capture-MS Homo sapiens
27 YWHAG 7532
Affinity Capture-MS Homo sapiens
28 PLXNA3 55558
Affinity Capture-MS Homo sapiens
29 AGTR1  
Two-hybrid Homo sapiens
30 CEPT1 10390
Affinity Capture-MS Homo sapiens
31 PGM2L1 283209
Affinity Capture-MS Homo sapiens
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