Gene description for Purb
Gene name purine rich element binding protein B
Gene symbol Purb
Other names/aliases pur-beta
Species Rattus norvegicus
 Database cross references - Purb
ExoCarta ExoCarta_498407
Vesiclepedia VP_498407
Entrez Gene 498407
UniProt Q68A21  
 Purb identified in sEVs derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Purb
Molecular Function
    mRNA regulatory element binding translation repressor activity GO:0000900 IDA
    RNA polymerase II transcription regulatory region sequence-specific DNA binding GO:0000977 IBA
    RNA polymerase II transcription regulatory region sequence-specific DNA binding GO:0000977 IEA
    RNA polymerase II transcription regulatory region sequence-specific DNA binding GO:0000977 ISO
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription repressor activity, RNA polymerase II-specific GO:0001227 IDA
    DNA-binding transcription repressor activity, RNA polymerase II-specific GO:0001227 IEA
    DNA-binding transcription repressor activity, RNA polymerase II-specific GO:0001227 ISO
    DNA-binding transcription repressor activity, RNA polymerase II-specific GO:0001227 ISS
    double-stranded DNA binding GO:0003690 ISO
    single-stranded DNA binding GO:0003697 IEA
    single-stranded DNA binding GO:0003697 ISO
    single-stranded DNA binding GO:0003697 ISS
    DNA-binding transcription factor activity GO:0003700 ISO
    RNA binding GO:0003723 ISO
    mRNA binding GO:0003729 IEA
    mRNA binding GO:0003729 ISO
    mRNA binding GO:0003729 ISS
    purine-rich negative regulatory element binding GO:0032422 IBA
    purine-rich negative regulatory element binding GO:0032422 IDA
    purine-rich negative regulatory element binding GO:0032422 IEA
    SMAD binding GO:0046332 IEA
    SMAD binding GO:0046332 ISO
    DNA-binding transcription factor binding GO:0140297 IEA
    DNA-binding transcription factor binding GO:0140297 ISO
    DNA-binding transcription factor binding GO:0140297 ISS
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    negative regulation of transcription by RNA polymerase II GO:0000122 ISO
    negative regulation of transcription by RNA polymerase II GO:0000122 ISO
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    negative regulation of translation GO:0017148 IEA
    negative regulation of DNA-templated transcription GO:0045892 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 ISS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
 Experiment description of studies that identified Purb in sEVs
1
Experiment ID 93
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|GAPDH|CD9|CD151
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [MALDI TOF]
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Purb
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Dao  
Affinity Capture-MS Rattus norvegicus
2 Mib1  
Affinity Capture-MS Rattus norvegicus
3 Fbxo21  
Affinity Capture-MS Rattus norvegicus
4 STXBP5L  
Affinity Capture-MS Homo sapiens
5 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml
 Pathways in which Purb is involved
No pathways found





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