Gene description for IER3IP1
Gene name immediate early response 3 interacting protein 1
Gene symbol IER3IP1
Other names/aliases HSPC039
MEDS
PRO2309
Species Homo sapiens
 Database cross references - IER3IP1
ExoCarta ExoCarta_51124
Vesiclepedia VP_51124
Entrez Gene 51124
HGNC 18550
MIM 609382
UniProt Q9Y5U9  
 IER3IP1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for IER3IP1
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    positive regulation of extracellular matrix constituent secretion GO:0003331 IMP
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IBA
    brain development GO:0007420 IDA
    protein transport GO:0015031 IEA
    organ growth GO:0035265 IDA
    positive regulation of protein secretion GO:0050714 IMP
    regulation of fibroblast apoptotic process GO:2000269 IMP
Subcellular Localization
    Golgi membrane GO:0000139 IBA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 IBA
    endoplasmic reticulum membrane GO:0005789 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    Golgi apparatus GO:0005794 IDA
    membrane GO:0016020 HDA
    COPII-coated ER to Golgi transport vesicle GO:0030134 IBA
 Experiment description of studies that identified IER3IP1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for IER3IP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDIPT 10423
Two-hybrid Homo sapiens
2 PTPRG 5793
Affinity Capture-MS Homo sapiens
3 PIGP  
Two-hybrid Homo sapiens
4 CST1  
Two-hybrid Homo sapiens
5 KCNA1  
Two-hybrid Homo sapiens
6 CYSTM1 84418
Co-fractionation Homo sapiens
7 FASN 2194
Positive Genetic Homo sapiens
8 CSNK2B 1460
Two-hybrid Homo sapiens
9 PLP1 5354
Two-hybrid Homo sapiens
10 TMEM14B  
Two-hybrid Homo sapiens
11 TSSC1 7260
Synthetic Lethality Homo sapiens
12 TK1 7083
Two-hybrid Homo sapiens
13 TMEM80  
Two-hybrid Homo sapiens
14 AIG1  
Two-hybrid Homo sapiens
15 ZFYVE27 118813
Two-hybrid Homo sapiens
16 TM4SF18  
Two-hybrid Homo sapiens
17 ADCY9 115
Affinity Capture-MS Homo sapiens
18 GNG5 2787
Affinity Capture-MS Homo sapiens
19 FCER1G 2207
Two-hybrid Homo sapiens
20 FAM3C 10447
Co-fractionation Homo sapiens
21 HERPUD2  
Two-hybrid Homo sapiens
22 TMEM128  
Two-hybrid Homo sapiens
23 RGS2  
Two-hybrid Homo sapiens
24 PEX12  
Two-hybrid Homo sapiens
25 TMEM86B  
Two-hybrid Homo sapiens
26 TMEM258  
Two-hybrid Homo sapiens
27 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
28 GOSR2 9570
Two-hybrid Homo sapiens
29 EBP  
Two-hybrid Homo sapiens
30 YIPF5 81555
Affinity Capture-MS Homo sapiens
31 JAGN1 84522
Two-hybrid Homo sapiens
32 SLC30A4 7782
Two-hybrid Homo sapiens
33 VPS13C 54832
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 TMEM167A 153339
Two-hybrid Homo sapiens
35 DPM3 54344
Two-hybrid Homo sapiens
36 KIAA1429 25962
Affinity Capture-MS Homo sapiens
37 CERS2 29956
Two-hybrid Homo sapiens
38 C19orf18 147685
Two-hybrid Homo sapiens
39 ACACA 31
Positive Genetic Homo sapiens
40 GPR42  
Two-hybrid Homo sapiens
41 MOV10 4343
Affinity Capture-RNA Homo sapiens
42 TMEM237 65062
Two-hybrid Homo sapiens
43 POMK  
Two-hybrid Homo sapiens
44 YIPF4 84272
Two-hybrid Homo sapiens
45 MOSPD3  
Two-hybrid Homo sapiens
46 GJB1 2705
Two-hybrid Homo sapiens
47 TMX2 51075
Two-hybrid Homo sapiens
48 CCDC53 51019
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 RER1 11079
Affinity Capture-MS Homo sapiens
50 UBQLN1 29979
Two-hybrid Homo sapiens
51 SCN3B  
Two-hybrid Homo sapiens
52 ELOVL4  
Two-hybrid Homo sapiens
53 TRHR  
Two-hybrid Homo sapiens
54 FAM210B  
Two-hybrid Homo sapiens
55 TMPPE  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which IER3IP1 is involved
No pathways found





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