Gene description for CHST15
Gene name carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15
Gene symbol CHST15
Other names/aliases BRAG
GALNAC4S-6ST
Species Homo sapiens
 Database cross references - CHST15
ExoCarta ExoCarta_51363
Vesiclepedia VP_51363
Entrez Gene 51363
HGNC 18137
MIM 608277
UniProt Q7LFX5  
 CHST15 identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for CHST15
Molecular Function
    protein binding GO:0005515 IPI
    3'-phosphoadenosine 5'-phosphosulfate binding GO:0050656 IDA
    N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase activity GO:0050659 IBA
    N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase activity GO:0050659 IDA
    N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase activity GO:0050659 TAS
Biological Process
    hexose biosynthetic process GO:0019319 IBA
    hexose biosynthetic process GO:0019319 IDA
    chondroitin sulfate biosynthetic process GO:0030206 TAS
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    membrane GO:0016020 NAS
 Experiment description of studies that identified CHST15 in sEVs
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for CHST15
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FKBP7 51661
Affinity Capture-MS Homo sapiens
2 CANX 821
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 NDUFAF2  
Affinity Capture-MS Homo sapiens
4 ILKAP 80895
Affinity Capture-MS Homo sapiens
5 FARS2 10667
Affinity Capture-MS Homo sapiens
6 SLC35E1 79939
Affinity Capture-MS Homo sapiens
7 KLHDC2  
Affinity Capture-MS Homo sapiens
8 HCK 3055
Affinity Capture-Western Homo sapiens
9 RABL6 55684
Affinity Capture-MS Homo sapiens
10 HIVEP1 3096
Affinity Capture-MS Homo sapiens
11 MARC1  
Affinity Capture-MS Homo sapiens
12 CCNB1 891
Affinity Capture-MS Homo sapiens
13 FAM69A 388650
Affinity Capture-MS Homo sapiens
14 LPGAT1 9926
Affinity Capture-MS Homo sapiens
15 SLC43A3 29015
Affinity Capture-MS Homo sapiens
16 PHF5A 84844
Affinity Capture-MS Homo sapiens
17 TJAP1 93643
Affinity Capture-MS Homo sapiens
18 MEOX2  
Two-hybrid Homo sapiens
19 EIF5A 1984
Affinity Capture-MS Homo sapiens
20 ERC1 23085
Affinity Capture-MS Homo sapiens
21 PLEKHG1  
Affinity Capture-MS Homo sapiens
22 PIN4 5303
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 CEPT1 10390
Affinity Capture-MS Homo sapiens
24 SLC25A19  
Affinity Capture-MS Homo sapiens
25 MORN5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 CASC3  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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