Gene description for BIN2
Gene name bridging integrator 2
Gene symbol BIN2
Other names/aliases BRAP-1
Species Homo sapiens
 Database cross references - BIN2
ExoCarta ExoCarta_51411
Vesiclepedia VP_51411
Entrez Gene 51411
HGNC 1053
MIM 605936
UniProt Q9UBW5  
 BIN2 identified in sEVs derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for BIN2
Molecular Function
    protein binding GO:0005515 IPI
    phospholipid binding GO:0005543 IBA
    phospholipid binding GO:0005543 IDA
Biological Process
    phagocytosis, engulfment GO:0006911 IBA
    phagocytosis, engulfment GO:0006911 IMP
    cell chemotaxis GO:0060326 IMP
    podosome assembly GO:0071800 IBA
    podosome assembly GO:0071800 IMP
    plasma membrane tubulation GO:0097320 IBA
    plasma membrane tubulation GO:0097320 IDA
Subcellular Localization
    phagocytic cup GO:0001891 IBA
    phagocytic cup GO:0001891 IDA
    podosome GO:0002102 IBA
    podosome GO:0002102 IDA
    extracellular region GO:0005576 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    cell cortex GO:0005938 IEA
    secretory granule lumen GO:0034774 TAS
    cell projection GO:0042995 IEA
    anchoring junction GO:0070161 IEA
    ficolin-1-rich granule lumen GO:1904813 TAS
 Experiment description of studies that identified BIN2 in sEVs
1
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for BIN2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 POT1  
Two-hybrid Homo sapiens
2 KIF1B 23095
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 TERF1 7013
Two-hybrid Homo sapiens
4 TINF2  
Two-hybrid Homo sapiens
5 HIST1H3A 8350
Cross-Linking-MS (XL-MS) Homo sapiens
6 EFEMP1 2202
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 CEBPA  
Protein-peptide Homo sapiens
8 BIN1 274
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 STK3 6788
Affinity Capture-Western Homo sapiens
10 AMPH 273
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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