Gene description for PHYH
Gene name phytanoyl-CoA 2-hydroxylase
Gene symbol PHYH
Other names/aliases LN1
LNAP1
PAHX
PHYH1
RD
Species Homo sapiens
 Database cross references - PHYH
ExoCarta ExoCarta_5264
Vesiclepedia VP_5264
Entrez Gene 5264
HGNC 8940
MIM 602026
UniProt O14832  
 PHYH identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for PHYH
Molecular Function
    protein binding GO:0005515 IPI
    ferrous iron binding GO:0008198 IDA
    carboxylic acid binding GO:0031406 IDA
    L-ascorbic acid binding GO:0031418 IDA
    phytanoyl-CoA dioxygenase activity GO:0048244 IBA
    phytanoyl-CoA dioxygenase activity GO:0048244 IDA
Biological Process
    fatty acid alpha-oxidation GO:0001561 IBA
    fatty acid alpha-oxidation GO:0001561 IDA
    2-oxoglutarate metabolic process GO:0006103 IDA
    isoprenoid metabolic process GO:0006720 IDA
    2-oxobutyrate catabolic process GO:0019606 IEA
    methyl-branched fatty acid metabolic process GO:0097089 IDA
Subcellular Localization
    peroxisome GO:0005777 IDA
    peroxisomal matrix GO:0005782 TAS
    cytosol GO:0005829 TAS
    9+0 non-motile cilium GO:0097731 IDA
 Experiment description of studies that identified PHYH in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PHYH
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 INADL 10207
Two-hybrid Homo sapiens
2 HSD17B4 3295
Affinity Capture-MS Homo sapiens
3 PBLD 64081
Co-fractionation Homo sapiens
4 GNPAT 8443
Affinity Capture-MS Homo sapiens
5 TP53 7157
Synthetic Growth Defect Homo sapiens
6 PEX7  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
7 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
8 MAGEA11  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
9 FAM9B  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
10 SIRPA 140885
Reconstituted Complex Homo sapiens
11 CLUH 23277
Affinity Capture-MS Homo sapiens
12 FKBP4 2288
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
13 APP 351
Reconstituted Complex Homo sapiens
14 WRAP73 49856
Affinity Capture-MS Homo sapiens
15 NANOS1 340719
Affinity Capture-MS Homo sapiens
16 ACOX1 51
Affinity Capture-MS Homo sapiens
17 C6orf226  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 PEX14 5195
Affinity Capture-MS Homo sapiens
19 PEX5 5830
Affinity Capture-MS Homo sapiens
20 COPS3 8533
Affinity Capture-MS Homo sapiens
21 AGPS 8540
Proximity Label-MS Homo sapiens
22 TOM1L1 10040
Two-hybrid Homo sapiens
23 F8 2157
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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