Experiment description of studies that identified Nap1l1 in sEVs

1 Experiment ID 907 MISEV standards ✔ EM Biophysical techniques ✔ Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a Enriched markers ✔ HSPA5 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." Journal name Proteomics Publication year 2023 Sample Colon cancer cells Sample name C26 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 210 MISEV standards ✔ EM Biophysical techniques ✔ CD81|FLOT1 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23260141     Organism Mus musculus Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration. Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL." Journal name Cell Publication year 2012 Sample Fibroblasts Sample name Normal-Fibroblasts (L cells) Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 15 MISEV standards ✔ EM Biophysical techniques ✔ CD63 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 17486113     Organism Mus musculus Homo sapiens Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO" Journal name NCB Publication year 2007 Sample Mast cells Sample name MC9 Bone marrow-derived mast cells HMC-1 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.11-1.21 g/mL Molecules identified in the study Protein mRNA miRNA Methods used in the study Mass spectrometry [MALDI TOF] Western blotting Microarray miRCURY LNA Array

4 Experiment ID 908 MISEV standards ✔ EM Biophysical techniques ✔ Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a Enriched markers ✔ HSPA5 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." Journal name Proteomics Publication year 2023 Sample T-cell lymphoma cells Sample name EL4 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for Nap1l1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 Kctd13   Affinity Capture-MS Mus musculus 2 Eed   Affinity Capture-MS Mus musculus 3 Ranbp3   Co-fractionation Mus musculus 4 Ranbp1 19385 Co-fractionation Mus musculus 5 Cops2 12848 Two-hybrid Mus musculus 6 Atg16l1   Affinity Capture-MS Mus musculus 7 Iqcb1   Affinity Capture-MS Mus musculus 8 Fancd2   Affinity Capture-MS Mus musculus View the network

Pathways in which Nap1l1 is involved

No pathways found