Gene description for CHPF2
Gene name chondroitin polymerizing factor 2
Gene symbol CHPF2
Other names/aliases CSGLCA-T
CSGlcAT
ChSy-3
chPF-2
Species Homo sapiens
 Database cross references - CHPF2
ExoCarta ExoCarta_54480
Vesiclepedia VP_54480
Entrez Gene 54480
HGNC 29270
MIM 608037
UniProt Q9P2E5  
 CHPF2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for CHPF2
Molecular Function
    glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity GO:0047238 IBA
    N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase activity GO:0050510 TAS
Biological Process
    chondroitin sulfate biosynthetic process GO:0030206 IBA
    chondroitin sulfate biosynthetic process GO:0030206 TAS
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    membrane GO:0016020 HDA
    Golgi cisterna membrane GO:0032580 IEA
 Experiment description of studies that identified CHPF2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CHPF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 P3H1 64175
Affinity Capture-MS Homo sapiens
2 CCL3  
Affinity Capture-MS Homo sapiens
3 LY6H  
Affinity Capture-MS Homo sapiens
4 C7orf34  
Affinity Capture-MS Homo sapiens
5 GPIHBP1  
Affinity Capture-MS Homo sapiens
6 MARCH3 115123
Affinity Capture-MS Homo sapiens
7 FMOD 2331
Affinity Capture-MS Homo sapiens
8 BTNL2  
Affinity Capture-MS Homo sapiens
9 TMEM106A  
Affinity Capture-MS Homo sapiens
10 NAAA  
Affinity Capture-MS Homo sapiens
11 PTCH1  
Affinity Capture-MS Homo sapiens
12 ECEL1  
Affinity Capture-MS Homo sapiens
13 LCN6  
Affinity Capture-MS Homo sapiens
14 FEN1 2237
Affinity Capture-MS Homo sapiens
15 FSTL4  
Affinity Capture-MS Homo sapiens
16 PLD6  
Affinity Capture-MS Homo sapiens
17 B3GNT3 10331
Affinity Capture-MS Homo sapiens
18 TMEM25  
Affinity Capture-MS Homo sapiens
19 GGH 8836
Affinity Capture-MS Homo sapiens
20 MARCH2  
Affinity Capture-MS Homo sapiens
21 DPEP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 PDGFRA 5156
Affinity Capture-MS Homo sapiens
23 GOLT1B 51026
Affinity Capture-MS Homo sapiens
24 PRG2  
Affinity Capture-MS Homo sapiens
25 MFAP4 4239
Affinity Capture-MS Homo sapiens
26 PI15  
Affinity Capture-MS Homo sapiens
27 PLD4  
Affinity Capture-MS Homo sapiens
28 LYZL1  
Affinity Capture-MS Homo sapiens
29 FBXO6 26270
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 ATP1B3 483
Affinity Capture-MS Homo sapiens
31 FCN3 8547
Affinity Capture-MS Homo sapiens
32 IL5RA  
Affinity Capture-MS Homo sapiens
33 LYZL6  
Affinity Capture-MS Homo sapiens
34 SFTPC  
Affinity Capture-MS Homo sapiens
35 EPHA7 2045
Affinity Capture-MS Homo sapiens
36 MSTN  
Affinity Capture-MS Homo sapiens
37 CLEC12B  
Affinity Capture-MS Homo sapiens
38 GAA 2548
Affinity Capture-MS Homo sapiens
39 C1QB 713
Affinity Capture-MS Homo sapiens
40 PLD3 23646
Affinity Capture-MS Homo sapiens
41 IDS  
Affinity Capture-MS Homo sapiens
42 SDF2L1 23753
Affinity Capture-MS Homo sapiens
43 IL12RB1  
Affinity Capture-MS Homo sapiens
44 CRISP2  
Affinity Capture-MS Homo sapiens
45 SCGB2A2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 C1orf54  
Affinity Capture-MS Homo sapiens
47 TRGV3  
Affinity Capture-MS Homo sapiens
48 ALPP 250
Affinity Capture-MS Homo sapiens
49 DNASE1L1 1774
Affinity Capture-MS Homo sapiens
50 MPPE1 65258
Affinity Capture-MS Homo sapiens
51 BRPF3  
Affinity Capture-MS Homo sapiens
52 ISLR 3671
Affinity Capture-MS Homo sapiens
53 Ptpn13  
Affinity Capture-MS Mus musculus
54 CHSY3 337876
Affinity Capture-MS Homo sapiens
55 FIBIN  
Affinity Capture-MS Homo sapiens
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