Gene description for RIC8B
Gene name RIC8 guanine nucleotide exchange factor B
Gene symbol RIC8B
Other names/aliases RIC8
hSyn
Species Homo sapiens
 Database cross references - RIC8B
ExoCarta ExoCarta_55188
Vesiclepedia VP_55188
Entrez Gene 55188
HGNC 25555
MIM 609147
UniProt Q9NVN3  
 RIC8B identified in sEVs derived from the following tissue/cell type
Neuroblastoma cells 25944692    
 Gene ontology annotations for RIC8B
Molecular Function
    G-protein alpha-subunit binding GO:0001965 IBA
    G-protein alpha-subunit binding GO:0001965 IDA
    guanyl-nucleotide exchange factor activity GO:0005085 IBA
    guanyl-nucleotide exchange factor activity GO:0005085 ISS
    protein folding chaperone GO:0044183 ISS
Biological Process
    protein folding GO:0006457 IEA
    G protein-coupled receptor signaling pathway GO:0007186 IBA
    G protein-coupled receptor signaling pathway GO:0007186 ISS
    sensory perception of smell GO:0007608 ISS
    regulation of G protein-coupled receptor signaling pathway GO:0008277 IDA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    cell cortex GO:0005938 IEA
 Experiment description of studies that identified RIC8B in sEVs
1
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for RIC8B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CEP41  
Affinity Capture-MS Homo sapiens
2 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
3 CAMKMT  
Affinity Capture-MS Homo sapiens
4 TFDP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 PPM1M  
Affinity Capture-MS Homo sapiens
6 RASL10A  
Affinity Capture-MS Homo sapiens
7 ZHX1-C8orf76  
Affinity Capture-MS Homo sapiens
8 XPO1 7514
Affinity Capture-MS Homo sapiens
9 NUBP2 10101
Affinity Capture-MS Homo sapiens
10 TUBAL3 79861
Affinity Capture-MS Homo sapiens
11 SULT1C4  
Affinity Capture-MS Homo sapiens
12 FAM109B  
Affinity Capture-MS Homo sapiens
13 ENSA 2029
Affinity Capture-MS Homo sapiens
14 CPNE9  
Affinity Capture-MS Homo sapiens
15 CHIA  
Affinity Capture-MS Homo sapiens
16 KLHDC2  
Protein-peptide Homo sapiens
Protein-peptide Homo sapiens
17 NAP1L1 4673
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 KLHL34  
Affinity Capture-MS Homo sapiens
19 OVOL2  
Affinity Capture-MS Homo sapiens
20 AARSD1 80755
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 OCIAD1 54940
Affinity Capture-MS Homo sapiens
22 LRP1 4035
Two-hybrid Homo sapiens
23 PPP4R1L  
Affinity Capture-MS Homo sapiens
24 GNAS 2778
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 VWA2 340706
Affinity Capture-MS Homo sapiens
26 TIGD5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 UBE2O 63893
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 ACTL6A 86
Affinity Capture-MS Homo sapiens
29 LINC01587  
Affinity Capture-MS Homo sapiens
30 PPARD  
Affinity Capture-MS Homo sapiens
31 EGFR 1956
Negative Genetic Homo sapiens
32 THBS3 7059
Affinity Capture-MS Homo sapiens
33 CYP2B6  
Affinity Capture-MS Homo sapiens
34 ABHD8  
Affinity Capture-MS Homo sapiens
35 GNAI2 2771
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 MEF2BNB  
Affinity Capture-MS Homo sapiens
37 UPP1 7378
Affinity Capture-MS Homo sapiens
38 MAGED2 10916
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 CASQ2 845
Affinity Capture-MS Homo sapiens
40 INTU  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which RIC8B is involved
No pathways found





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