Gene description for TMEM165
Gene name transmembrane protein 165
Gene symbol TMEM165
Other names/aliases CDG2K
FT27
GDT1
TMPT27
TPARL
Species Homo sapiens
 Database cross references - TMEM165
ExoCarta ExoCarta_55858
Vesiclepedia VP_55858
Entrez Gene 55858
HGNC 30760
MIM 614726
UniProt Q9HC07  
 TMEM165 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 34887515    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for TMEM165
Molecular Function
    manganese ion transmembrane transporter activity GO:0005384 IBA
    calcium ion transmembrane transporter activity GO:0015085 IBA
    antiporter activity GO:0015297 IEA
Biological Process
    protein N-linked glycosylation GO:0006487 IMP
    calcium ion transport GO:0006816 IDA
    manganese ion transport GO:0006828 IDA
    intracellular calcium ion homeostasis GO:0006874 IGI
    Golgi calcium ion homeostasis GO:0032468 IBA
    Golgi calcium ion transport GO:0032472 IBA
    Golgi calcium ion transport GO:0032472 IDA
    regulation of lysosomal lumen pH GO:0035751 IMP
    calcium ion transmembrane transport GO:0070588 IBA
    manganese ion transmembrane transport GO:0071421 IBA
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    lysosomal membrane GO:0005765 IDA
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 IDA
    endosome membrane GO:0010008 IDA
    trans-Golgi network membrane GO:0032588 IDA
    cis-Golgi network membrane GO:0033106 ISS
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified TMEM165 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
9
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TMEM165
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CLPP 8192
Proximity Label-MS Homo sapiens
2 VPS35 55737
Synthetic Lethality Homo sapiens
3 SMC3 9126
Affinity Capture-MS Homo sapiens
4 Rab5c 19345
Affinity Capture-MS Mus musculus
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 Prkci  
Affinity Capture-MS Mus musculus
7 CYB5B 80777
Co-fractionation Homo sapiens
8 Tubg1 103733
Affinity Capture-MS Mus musculus
9 EBAG9 9166
Proximity Label-MS Homo sapiens
10 USP43  
Affinity Capture-MS Homo sapiens
11 FAM20C 56975
Affinity Capture-MS Homo sapiens
12 FBXL6  
Affinity Capture-MS Homo sapiens
13 TP53 7157
Affinity Capture-MS Homo sapiens
14 NR3C1 2908
Proximity Label-MS Homo sapiens
15 ARPC2 10109
Synthetic Lethality Homo sapiens
16 CALU 813
Co-fractionation Homo sapiens
17 GORASP2 26003
Affinity Capture-MS Homo sapiens
18 REL 5966
Two-hybrid Homo sapiens
19 VSIG1  
Affinity Capture-MS Homo sapiens
20 TRIM66  
Affinity Capture-MS Homo sapiens
21 GPR182  
Affinity Capture-MS Homo sapiens
22 BRD8 10902
Affinity Capture-MS Homo sapiens
23 SLC35E1 79939
Affinity Capture-MS Homo sapiens
24 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 SMC6  
Affinity Capture-MS Homo sapiens
26 CNOT2  
Affinity Capture-MS Homo sapiens
27 GJD3  
Proximity Label-MS Homo sapiens
28 UBE4B 10277
Synthetic Lethality Homo sapiens
29 GPR107 57720
Affinity Capture-MS Homo sapiens
30 B3GALT4  
Affinity Capture-MS Homo sapiens
31 AMFR 267
Affinity Capture-MS Homo sapiens
32 MTHFD1 4522
Co-fractionation Homo sapiens
33 DDX58 23586
Affinity Capture-RNA Homo sapiens
34 FFAR1  
Affinity Capture-MS Homo sapiens
35 ELOVL5 60481
Proximity Label-MS Homo sapiens
36 CDK1 983
Affinity Capture-MS Homo sapiens
37 Evpl  
Affinity Capture-MS Mus musculus
38 PDE2A  
Affinity Capture-MS Homo sapiens
39 RPS26 6231
Co-fractionation Homo sapiens
40 KIAA1429 25962
Affinity Capture-MS Homo sapiens
41 WDR62  
Synthetic Lethality Homo sapiens
42 FASN 2194
Negative Genetic Homo sapiens
43 MAPK6  
Affinity Capture-MS Homo sapiens
44 AHSP  
Two-hybrid Homo sapiens
45 RAB2A 5862
Proximity Label-MS Homo sapiens
46 UBXN8  
Affinity Capture-MS Homo sapiens
47 Klhl21  
Affinity Capture-MS Mus musculus
48 NIN 51199
Proximity Label-MS Homo sapiens
49 ERRFI1 54206
Two-hybrid Homo sapiens
50 SPPL2B 56928
Affinity Capture-MS Homo sapiens
51 SPRTN  
Affinity Capture-MS Homo sapiens
52 Snw1  
Affinity Capture-MS Mus musculus
53 B3GAT1  
Proximity Label-MS Homo sapiens
54 CDK2AP1  
Affinity Capture-MS Homo sapiens
55 LDLR 3949
Negative Genetic Homo sapiens
56 GPATCH8  
Affinity Capture-MS Homo sapiens
57 MRPS36 92259
Cross-Linking-MS (XL-MS) Homo sapiens
58 RPLP1 6176
Co-fractionation Homo sapiens
59 RAB5C 5878
Proximity Label-MS Homo sapiens
60 SERBP1 26135
Affinity Capture-MS Homo sapiens
61 STX5 6811
Affinity Capture-MS Homo sapiens
62 SLC1A1 6505
Affinity Capture-MS Homo sapiens
63 RPA2 6118
Proximity Label-MS Homo sapiens
64 ATP2C1 27032
Synthetic Lethality Homo sapiens
65 RPA3 6119
Proximity Label-MS Homo sapiens
66 RNF139  
Affinity Capture-MS Homo sapiens
67 PPME1 51400
Synthetic Lethality Homo sapiens
68 PTAR1 375743
Synthetic Lethality Homo sapiens
Synthetic Lethality Homo sapiens
69 COX4I1 1327
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TMEM165 is involved
No pathways found





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