Gene description for Pitpnb
Gene name phosphatidylinositol transfer protein, beta
Gene symbol Pitpnb
Other names/aliases PI-TP-beta
Species Mus musculus
 Database cross references - Pitpnb
ExoCarta ExoCarta_56305
Vesiclepedia VP_56305
Entrez Gene 56305
UniProt P53811  
 Pitpnb identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Pitpnb
Molecular Function
    phospholipid binding GO:0005543 ISA
    phosphatidylcholine transporter activity GO:0008525 IBA
    phosphatidylcholine transporter activity GO:0008525 ISO
    phosphatidylinositol transfer activity GO:0008526 IBA
    phosphatidylinositol transfer activity GO:0008526 ISO
    phosphatidylinositol transfer activity GO:0008526 ISS
    phosphatidylcholine binding GO:0031210 IBA
    phosphatidylcholine binding GO:0031210 ISO
    phosphatidylinositol binding GO:0035091 IBA
    phosphatidylinositol binding GO:0035091 ISO
    phosphatidylcholine transfer activity GO:0120019 ISO
    phosphatidylcholine transfer activity GO:0120019 ISS
    sphingomyelin transfer activity GO:0140338 ISS
Biological Process
    in utero embryonic development GO:0001701 IMP
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 ISO
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 ISS
    phospholipid transport GO:0015914 IEA
    phospholipid transport GO:0015914 ISO
    intermembrane lipid transfer GO:0120009 IEA
Subcellular Localization
    Golgi membrane GO:0000139 ISO
    Golgi membrane GO:0000139 ISS
    cytoplasm GO:0005737 IBA
    endoplasmic reticulum membrane GO:0005789 ISO
    endoplasmic reticulum membrane GO:0005789 ISS
    Golgi apparatus GO:0005794 IDA
 Experiment description of studies that identified Pitpnb in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Pitpnb
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Lmna 16905
Affinity Capture-MS Mus musculus
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