Gene description for Vps29
Gene name vacuolar protein sorting 29 (S. pombe)
Gene symbol Vps29
Other names/aliases 2010015D08Rik
AW049835
PEP11
Species Mus musculus
 Database cross references - Vps29
ExoCarta ExoCarta_56433
Vesiclepedia VP_56433
Entrez Gene 56433
UniProt Q9QZ88  
 Vps29 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Vps29
Molecular Function
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    intracellular protein transport GO:0006886 IBA
    Golgi to vacuole transport GO:0006896 ISS
    protein transport GO:0015031 IEA
    endocytic recycling GO:0032456 ISO
    endocytic recycling GO:0032456 ISS
    retrograde transport, endosome to Golgi GO:0042147 IBA
    retrograde transport, endosome to Golgi GO:0042147 IEA
Subcellular Localization
    endosome GO:0005768 IBA
    endosome GO:0005768 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    endosome membrane GO:0010008 IEA
    retromer complex GO:0030904 IBA
    retromer complex GO:0030904 IDA
    retromer complex GO:0030904 ISO
    retromer, cargo-selective complex GO:0030906 IEA
    retromer, cargo-selective complex GO:0030906 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
 Experiment description of studies that identified Vps29 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Vps29
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Nudc 18221
Co-fractionation Mus musculus
2 Snx2 67804
Co-fractionation Mus musculus
3 Snx5 69178
Co-fractionation Mus musculus
4 Shmt1 20425
Co-fractionation Mus musculus
5 Tbcd  
Co-fractionation Mus musculus
6 Eed  
Affinity Capture-MS Mus musculus
7 Gtf2f1  
Co-fractionation Mus musculus
8 Usp7  
Co-fractionation Mus musculus
9 Rab7 19349
Co-fractionation Mus musculus
10 Vps26b  
Co-fractionation Mus musculus
11 Pus1  
Co-fractionation Mus musculus
12 Tbc1d5  
Co-fractionation Mus musculus
13 Vps35 65114
Co-fractionation Mus musculus
14 Atg12  
Negative Genetic Mus musculus
15 Sstr3  
Proximity Label-MS Mus musculus
16 Snx6 72183
Co-fractionation Mus musculus
17 Fitm2  
Positive Genetic Mus musculus
18 Snx1  
Co-fractionation Mus musculus
19 Vps26a 30930
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Vps29 is involved
PathwayEvidenceSource
Signal Transduction IEA Reactome
Signaling by WNT IEA Reactome
WNT ligand biogenesis and trafficking IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here