Gene description for ASAH2
Gene name N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2
Gene symbol ASAH2
Other names/aliases BCDase
HNAC1
LCDase
N-CDase
NCDase
Species Homo sapiens
 Database cross references - ASAH2
ExoCarta ExoCarta_56624
Vesiclepedia VP_56624
Entrez Gene 56624
HGNC 18860
MIM 611202
UniProt Q9NR71  
 ASAH2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ASAH2
Molecular Function
    calcium ion binding GO:0005509 IDA
    zinc ion binding GO:0008270 IDA
    N-acylsphingosine amidohydrolase activity GO:0017040 IBA
    N-acylsphingosine amidohydrolase activity GO:0017040 IDA
    N-acylsphingosine amidohydrolase activity GO:0017040 IMP
Biological Process
    sphingosine metabolic process GO:0006670 IDA
    sphingosine metabolic process GO:0006670 IMP
    ceramide metabolic process GO:0006672 IDA
    apoptotic process GO:0006915 IEA
    regulation of mitotic cell cycle GO:0007346 IMP
    long-chain fatty acid biosynthetic process GO:0042759 IBA
    lipid digestion GO:0044241 ISS
    sphingosine biosynthetic process GO:0046512 IBA
    sphingosine biosynthetic process GO:0046512 IMP
    ceramide biosynthetic process GO:0046513 IDA
    ceramide biosynthetic process GO:0046513 IMP
    ceramide catabolic process GO:0046514 IBA
    ceramide catabolic process GO:0046514 IDA
    ceramide catabolic process GO:0046514 IMP
    cellular response to cytokine stimulus GO:0071345 IDA
    negative regulation of apoptotic signaling pathway GO:2001234 IMP
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    extracellular region GO:0005576 IBA
    mitochondrion GO:0005739 IDA
    Golgi apparatus GO:0005794 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISS
    caveola GO:0005901 ISS
    membrane raft GO:0045121 ISS
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified ASAH2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ASAH2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MYH3 4621
Affinity Capture-MS Homo sapiens
2 MYH7 4625
Affinity Capture-MS Homo sapiens
3 MYH4  
Affinity Capture-MS Homo sapiens
4 MYH1  
Affinity Capture-MS Homo sapiens
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