Experiment description of studies that identified SPIRE1 in sEVs

1 Experiment ID 488 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

2 Experiment ID 224 MISEV standards ✔ EM|AFM Biophysical techniques ✔ Alix|TSG101|CD63|CD81 Enriched markers ✔ GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25944692     Organism Homo sapiens Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S" Journal name Oncotarget Publication year 2015 Sample Neuroblastoma cells Sample name SH-SY5Y Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient Flotation density 1.10 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

Protein-protein interactions for SPIRE1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 YWHAH 7533 Affinity Capture-MS Homo sapiens 2 SFN 2810 Affinity Capture-MS Homo sapiens 3 YWHAQ 10971 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 4 ACTB 60 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 5 ACTC1 70 Affinity Capture-MS Homo sapiens 6 YWHAG 7532 Affinity Capture-MS Homo sapiens 7 MTCH2 23788 Proximity Label-MS Homo sapiens 8 PLEKHA4 57664 Affinity Capture-MS Homo sapiens 9 FBXW11   Affinity Capture-MS Homo sapiens 10 YWHAZ 7534 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 11 YWHAE 7531 Affinity Capture-MS Homo sapiens 12 BCL7A   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 13 ACTG1 71 Affinity Capture-MS Homo sapiens 14 CBY1   Affinity Capture-MS Homo sapiens 15 BBS4 585 Two-hybrid Homo sapiens 16 TMOD3 29766 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 17 YWHAB 7529 Affinity Capture-MS Homo sapiens 18 BCL7C   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens View the network

Pathways in which SPIRE1 is involved

No pathways found