Gene description for TBX20
Gene name T-box 20
Gene symbol TBX20
Other names/aliases ASD4
Species Homo sapiens
 Database cross references - TBX20
ExoCarta ExoCarta_57057
Vesiclepedia VP_57057
Entrez Gene 57057
HGNC 11598
MIM 606061
UniProt Q9UMR3  
 TBX20 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for TBX20
Molecular Function
    RNA polymerase II transcription regulatory region sequence-specific DNA binding GO:0000977 IDA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IDA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IEA
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 ISS
    sequence-specific double-stranded DNA binding GO:1990837 IDA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 ISS
    branching involved in blood vessel morphogenesis GO:0001569 IEA
    endoderm formation GO:0001706 ISS
    cell fate specification GO:0001708 IBA
    heart looping GO:0001947 IBA
    heart looping GO:0001947 ISS
    embryonic heart tube morphogenesis GO:0003143 ISS
    outflow tract septum morphogenesis GO:0003148 IMP
    atrioventricular valve development GO:0003171 ISS
    tricuspid valve development GO:0003175 ISS
    aortic valve morphogenesis GO:0003180 IMP
    pulmonary valve formation GO:0003193 ISS
    endocardial cushion morphogenesis GO:0003203 ISS
    cardiac chamber formation GO:0003207 ISS
    cardiac right ventricle morphogenesis GO:0003215 ISS
    endocardial cushion formation GO:0003272 ISS
    cardiac septum development GO:0003279 ISS
    pericardium morphogenesis GO:0003344 ISS
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    muscle contraction GO:0006936 IEA
    blood circulation GO:0008015 IEA
    cell population proliferation GO:0008283 IEA
    dorsal/ventral pattern formation GO:0009953 ISS
    positive regulation of epithelial to mesenchymal transition GO:0010718 IEA
    mesenchymal cell development GO:0014031 ISS
    visceral motor neuron differentiation GO:0021524 ISS
    positive regulation of BMP signaling pathway GO:0030513 ISS
    foramen ovale closure GO:0035922 IMP
    atrioventricular canal development GO:0036302 ISS
    embryonic heart tube elongation GO:0036306 IEA
    positive regulation of apoptotic process GO:0043065 IEA
    negative regulation of DNA-templated transcription GO:0045892 ISS
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    lateral mesoderm formation GO:0048370 ISS
    cardiac muscle tissue morphogenesis GO:0055008 ISS
    positive regulation of cardiac muscle cell proliferation GO:0060045 ISS
    negative regulation of SMAD protein signal transduction GO:0060392 IEA
    atrial septum morphogenesis GO:0060413 IMP
    pulmonary vein morphogenesis GO:0060577 IMP
    positive regulation of cell cycle process GO:0090068 IEA
    motor neuron migration GO:0097475 IEA
Subcellular Localization
    chromatin GO:0000785 IBA
    chromatin GO:0000785 ISA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 ISS
 Experiment description of studies that identified TBX20 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for TBX20
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PHF13  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 PDZRN3 23024
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
3 ZSCAN1  
Two-hybrid Homo sapiens
4 FAM212B  
Affinity Capture-MS Homo sapiens
5 SLC19A2 10560
Affinity Capture-MS Homo sapiens
6 AGO4  
Affinity Capture-MS Homo sapiens
7 TLE1 7088
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 KCTD10 83892
Affinity Capture-MS Homo sapiens
9 DNASE1L2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 TLE3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 XRCC6 2547
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
12 LENG1  
Affinity Capture-MS Homo sapiens
13 SLC25A21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 BLVRA 644
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 XRCC5 7520
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
16 TBX18  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 CTPS2 56474
Affinity Capture-MS Homo sapiens
18 TLE4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TBX20 is involved
PathwayEvidenceSource
Cardiogenesis IEA Reactome
Developmental Biology IEA Reactome





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