Gene description for TMEM27
Gene name transmembrane protein 27
Gene symbol TMEM27
Other names/aliases NX-17
NX17
Species Homo sapiens
 Database cross references - TMEM27
ExoCarta ExoCarta_57393
Vesiclepedia VP_57393
Entrez Gene 57393
HGNC 29437
MIM 300631
UniProt Q9HBJ8  
 TMEM27 identified in sEVs derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Urine 19056867    
 Gene ontology annotations for TMEM27
Molecular Function
    protein binding GO:0005515 IPI
    protein homodimerization activity GO:0042803 IDA
Biological Process
    calcium-ion regulated exocytosis GO:0017156 IDA
    regulation of transmembrane transporter activity GO:0022898 IGI
    SNARE complex assembly GO:0035493 IDA
    insulin secretion involved in cellular response to glucose stimulus GO:0035773 IDA
    positive regulation of amino acid transport GO:0051957 IBA
    positive regulation of L-proline import across plasma membrane GO:1905737 IGI
Subcellular Localization
    cytoplasm GO:0005737 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    brush border membrane GO:0031526 IEA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified TMEM27 in sEVs
1
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for TMEM27
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 OLFM4 10562
Two-hybrid Homo sapiens
2 SMCO4  
Two-hybrid Homo sapiens
3 RBM18  
Two-hybrid Homo sapiens
4 UBE2J1 51465
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which TMEM27 is involved
PathwayEvidenceSource
Insulin processing IEA Reactome
Metabolism of proteins IEA Reactome
Peptide hormone metabolism IEA Reactome





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