Gene description for SLC12A5
Gene name solute carrier family 12 (potassium/chloride transporter), member 5
Gene symbol SLC12A5
Other names/aliases KCC2
Species Homo sapiens
 Database cross references - SLC12A5
ExoCarta ExoCarta_57468
Vesiclepedia VP_57468
Entrez Gene 57468
HGNC 13818
MIM 606726
UniProt Q9H2X9  
 SLC12A5 identified in sEVs derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for SLC12A5
Molecular Function
    ammonium channel activity GO:0008519 IEA
    chloride transmembrane transporter activity GO:0015108 IDA
    potassium:chloride symporter activity GO:0015379 IBA
    potassium:chloride symporter activity GO:0015379 IDA
    potassium:chloride symporter activity GO:0015379 TAS
    protein kinase binding GO:0019901 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    monoatomic ion transport GO:0006811 IDA
    monoatomic ion transport GO:0006811 TAS
    cell volume homeostasis GO:0006884 IBA
    hypotonic response GO:0006971 IDA
    chemical synaptic transmission GO:0007268 IBA
    learning GO:0007612 IEA
    response to xenobiotic stimulus GO:0009410 IEA
    intracellular chloride ion homeostasis GO:0030644 IDA
    multicellular organism growth GO:0035264 IEA
    thermosensory behavior GO:0040040 IEA
    intracellular pH reduction GO:0051452 IEA
    chloride ion homeostasis GO:0055064 IBA
    potassium ion homeostasis GO:0055075 IBA
    dendritic spine development GO:0060996 IDA
    ammonium transmembrane transport GO:0072488 IEA
    postsynaptic neurotransmitter receptor diffusion trapping GO:0098970 IEA
    regulation of postsynapse assembly GO:0150052 IEA
    chloride transmembrane transport GO:1902476 IBA
    potassium ion import across plasma membrane GO:1990573 IBA
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 IDA
    dendrite membrane GO:0032590 IEA
    neuron projection GO:0043005 IDA
    neuronal cell body GO:0043025 IDA
    neuronal cell body GO:0043025 ISS
    perikaryon GO:0043204 IEA
    cell periphery GO:0071944 IDA
    cell periphery GO:0071944 ISS
    glutamatergic synapse GO:0098978 IEA
    postsynaptic specialization membrane GO:0099634 IEA
 Experiment description of studies that identified SLC12A5 in sEVs
1
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for SLC12A5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GPM6A 2823
Affinity Capture-MS Homo sapiens
2 CCR4 1233
Two-hybrid Homo sapiens
3 F2RL1  
Two-hybrid Homo sapiens
4 DGUOK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 NUFIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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