Gene description for PTPRB
Gene name protein tyrosine phosphatase, receptor type, B
Gene symbol PTPRB
Other names/aliases HPTP-BETA
HPTPB
PTPB
R-PTP-BETA
VEPTP
Species Homo sapiens
 Database cross references - PTPRB
ExoCarta ExoCarta_5787
Vesiclepedia VP_5787
Entrez Gene 5787
HGNC 9665
MIM 176882
UniProt P23467  
 PTPRB identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for PTPRB
Molecular Function
    non-membrane spanning protein tyrosine phosphatase activity GO:0004726 IEA
    transmembrane receptor protein tyrosine phosphatase activity GO:0005001 TAS
    protein binding GO:0005515 IPI
    protein tyrosine phosphatase activity, metal-dependent GO:0030946 IEA
    cadherin binding GO:0045296 IBA
    cadherin binding GO:0045296 IPI
    histone H2AXY142 phosphatase activity GO:0140793 IEA
Biological Process
    angiogenesis GO:0001525 IBA
    osteoblast differentiation GO:0001649 IDA
    chromatin remodeling GO:0006338 IEA
    protein dephosphorylation GO:0006470 TAS
    phosphate-containing compound metabolic process GO:0006796 TAS
    glial cell migration GO:0008347 IEA
    dephosphorylation GO:0016311 IDA
Subcellular Localization
    plasma membrane GO:0005886 IC
    plasma membrane GO:0005886 TAS
    specific granule membrane GO:0035579 TAS
    receptor complex GO:0043235 IBA
    receptor complex GO:0043235 IDA
    tertiary granule membrane GO:0070821 TAS
 Experiment description of studies that identified PTPRB in sEVs
1
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PTPRB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RRP8  
Affinity Capture-MS Homo sapiens
2 PTPRG 5793
Proximity Label-MS Homo sapiens
3 PTN  
Co-localization Homo sapiens
4 RPL26L1 51121
Affinity Capture-MS Homo sapiens
5 NRCAM  
Reconstituted Complex Homo sapiens
6 NOC3L 64318
Affinity Capture-MS Homo sapiens
7 VEGFA 7422
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
8 RABEP2 79874
Proximity Label-MS Homo sapiens
9 BRIX1 55299
Affinity Capture-MS Homo sapiens
10 CDH5  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
11 DDX31  
Affinity Capture-MS Homo sapiens
12 LGALS1 3956
Affinity Capture-MS Homo sapiens
13 DDX21 9188
Affinity Capture-MS Homo sapiens
14 EGFR 1956
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
15 RBM28 55131
Affinity Capture-MS Homo sapiens
16 MAGI3 260425
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
17 PTPRS 5802
Affinity Capture-MS Homo sapiens
18 CNTN1 1272
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
19 HP1BP3 50809
Affinity Capture-MS Homo sapiens
20 GLYR1 84656
Affinity Capture-MS Homo sapiens
21 GHR  
Reconstituted Complex Homo sapiens
22 RBM34  
Affinity Capture-MS Homo sapiens
23 FTSJ3 117246
Affinity Capture-MS Homo sapiens
24 GOPC 57120
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PTPRB is involved
PathwayEvidenceSource
Immune System TAS Reactome
Innate Immune System TAS Reactome
Neutrophil degranulation TAS Reactome





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