Gene description for Cd274
Gene name CD274 antigen
Gene symbol Cd274
Other names/aliases A530045L16Rik
B7h1
Pdcd1l1
Pdcd1lg1
Pdl1
Species Mus musculus
 Database cross references - Cd274
ExoCarta ExoCarta_60533
Vesiclepedia VP_60533
Entrez Gene 60533
UniProt Q9EP73  
 Cd274 identified in sEVs derived from the following tissue/cell type
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Melanoma cells 30089911    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
 Gene ontology annotations for Cd274
Molecular Function
    transcription coactivator activity GO:0003713 IEA
    transcription coactivator activity GO:0003713 ISO
    protein binding GO:0005515 IPI
    receptor ligand activity GO:0048018 IEA
    receptor ligand activity GO:0048018 ISO
Biological Process
    adaptive immune response GO:0002250 IEA
    negative regulation of T cell mediated immune response to tumor cell GO:0002841 ISO
    negative regulation of T cell mediated immune response to tumor cell GO:0002841 ISS
    immune response GO:0006955 IBA
    immune response GO:0006955 ISO
    signal transduction GO:0007165 ISO
    cell surface receptor signaling pathway GO:0007166 IBA
    cell surface receptor signaling pathway GO:0007166 IEA
    cell surface receptor signaling pathway GO:0007166 ISO
    positive regulation of cell migration GO:0030335 IEA
    positive regulation of cell migration GO:0030335 ISO
    T cell costimulation GO:0031295 IBA
    T cell costimulation GO:0031295 IEA
    T cell costimulation GO:0031295 ISO
    negative regulation of type II interferon production GO:0032689 IEA
    negative regulation of type II interferon production GO:0032689 ISO
    negative regulation of interleukin-10 production GO:0032693 IEA
    negative regulation of interleukin-10 production GO:0032693 ISO
    positive regulation of interleukin-10 production GO:0032733 IEA
    positive regulation of interleukin-10 production GO:0032733 ISO
    response to cytokine GO:0034097 IEA
    response to cytokine GO:0034097 ISO
    TRIF-dependent toll-like receptor signaling pathway GO:0035666 IEA
    TRIF-dependent toll-like receptor signaling pathway GO:0035666 ISO
    positive regulation of T cell proliferation GO:0042102 IBA
    positive regulation of T cell proliferation GO:0042102 IEA
    positive regulation of T cell proliferation GO:0042102 ISO
    negative regulation of T cell proliferation GO:0042130 IBA
    negative regulation of T cell proliferation GO:0042130 IDA
    positive regulation of DNA-templated transcription GO:0045893 IEA
    negative regulation of activated T cell proliferation GO:0046007 IEA
    negative regulation of activated T cell proliferation GO:0046007 ISO
    cellular response to lipopolysaccharide GO:0071222 IBA
    negative regulation of tumor necrosis factor superfamily cytokine production GO:1903556 IEA
    negative regulation of tumor necrosis factor superfamily cytokine production GO:1903556 ISO
    positive regulation of activated CD8-positive, alpha-beta T cell apoptotic process GO:1905404 IEA
    positive regulation of activated CD8-positive, alpha-beta T cell apoptotic process GO:1905404 ISO
    negative regulation of CD4-positive, alpha-beta T cell proliferation GO:2000562 IEA
    negative regulation of CD4-positive, alpha-beta T cell proliferation GO:2000562 ISO
    negative regulation of CD8-positive, alpha-beta T cell activation GO:2001186 ISO
    negative regulation of CD8-positive, alpha-beta T cell activation GO:2001186 ISS
Subcellular Localization
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    plasma membrane GO:0005886 ISO
    external side of plasma membrane GO:0009897 IBA
    external side of plasma membrane GO:0009897 IDA
    cell surface GO:0009986 ISO
    actin cytoskeleton GO:0015629 IEA
    actin cytoskeleton GO:0015629 ISO
    early endosome membrane GO:0031901 ISO
    early endosome membrane GO:0031901 ISS
    recycling endosome membrane GO:0055038 ISO
    recycling endosome membrane GO:0055038 ISS
    extracellular exosome GO:0070062 IEA
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Cd274 in sEVs
1
Experiment ID 552
MISEV standards
✘ Biophysical techniques
✔
Cd63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Mus musculus
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name LLC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
2
Experiment ID 553
MISEV standards
✘ Biophysical techniques
✔
Cd63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Mus musculus
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name LLC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
3
Experiment ID 968
MISEV standards
✘ Biophysical techniques
✔
Tsg101|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Mus musculus
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
4
Experiment ID 1163
MISEV standards
✘ Biophysical techniques
✔
Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
5
Experiment ID 1164
MISEV standards
✘ Biophysical techniques
✔
Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name C57BL/6 bearing B16-F10 tumour
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
6
Experiment ID 1165
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
7
Experiment ID 1166
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
8
Experiment ID 1167
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
9
Experiment ID 1168
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
10
Experiment ID 1169
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name C57BL/6 bearing B16-F10 tumour
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
11
Experiment ID 1170
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name C57BL/6 bearing B16-F10 tumour
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
12
Experiment ID 1171
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name C57BL/6 bearing B16-F10 tumour
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
13
Experiment ID 1172
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name C57BL/6 bearing B16-F10 tumour
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
14
Experiment ID 1173
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
15
Experiment ID 1174
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
16
Experiment ID 1175
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
17
Experiment ID 1176
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
18
Experiment ID 1177
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
19
Experiment ID 1178
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
20
Experiment ID 1179
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
21
Experiment ID 1180
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
 Protein-protein interactions for Cd274
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Anxa1 16952
Protein-peptide Mus musculus
View the network image/svg+xml
 Pathways in which Cd274 is involved
PathwayEvidenceSource
Adaptive Immune System IEA Reactome
Costimulation by the CD28 family IEA Reactome
Immune System IEA Reactome
PD-1 signaling IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here