Experiment description of studies that identified NECAB3 in sEVs

1 Experiment ID 234 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-C3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

2 Experiment ID 237 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocytes Sample name MIHA Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RNA Sequencing

Protein-protein interactions for NECAB3

Protein Interactor ExoCarta ID Identification method PubMed Species 1 APP 351 Reconstituted Complex Homo sapiens 2 APEX1 328 Affinity Capture-RNA Homo sapiens 3 IKBKG 8517 Reconstituted Complex Homo sapiens 4 NECAB2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 5 APBA3   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 6 MYC   Dosage Lethality Homo sapiens 7 NECAB1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 8 APBA2 321 Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens View the network

Pathways in which NECAB3 is involved

No pathways found